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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/17804

Title: Microdroplet-based multiplex PCR on chip to detect foodborne bacteria producing biogenic amines
Authors: Sciancalepore, Anna G.
Mele, Elisa
Arcadio, Valentina
Reddavide, Francesco
Grieco, Francesco
Spano, Giuseppe
Lucas, Patrick
Mita, Giovanni
Pisignano, Dario
Keywords: Biogenic amines
Food safety
Issue Date: 2013
Publisher: © Elsevier Ltd
Citation: SCIANCALEPORE, A.G. ... et al, 2013. Microdroplet-based multiplex PCR on chip to detect foodborne bacteria producing biogenic amines. Food Microbiology, 35 (1), pp. 10 - 14.
Abstract: The development of fast, reliable and culture-independent molecular tools to detect bacteria producing biogenic amines deserves the attention of research and ultimately of the food industry in order to protect consumers’ health. Here we present the application of a simple, low-cost, fast and sensitive method to perform microdroplet-based multiplex PCR, directly on a food matrix, for the simultaneous detection of bacterial genes involved in biogenic amine biosynthesis. After inoculating wine with Lactobacillus brevis IOEB 9809, cell lysis and DNA amplification are performed in one single step, without preliminary nucleic acid extraction or purification treatments. The assay is performed in about 30 min, requiring 150 nL of starting sample and it enables the detection of down to 15 bacterial cells. With respect to traditional culture techniques, the speed, the simplicity and the cheapness of this procedure allow an effective monitoring of microbial cells during food-making and processing.
Description: This article is closed access.
Sponsor: G.S. and P.L. were supported by the European Union project BIAMFOOD/211441 (Controlling Biogenic Amines in Traditional Food Fermentations in Regional Europe).
Version: Published
DOI: 10.1016/j.fm.2013.02.010
URI: https://dspace.lboro.ac.uk/2134/17804
Publisher Link: http://dx.doi.org/10.1016/j.fm.2013.02.010
ISSN: 0740-0020
Appears in Collections:Closed Access (Materials)

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