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Title: A bioartificial renal tubule device embedding human renal stem/progenitor cells
Authors: Sciancalepore, Anna G.
Sallustio, F.
Girardo, Salvatore
Gioia Passione, L.
Camposeo, Andrea
Mele, Elisa
Di Lorenzo, M.
Costantino, V.
Schena, F.P.
Pisignano, Dario
Issue Date: 2014
Publisher: Public Library of Science / © The Authors
Citation: SCIANCALEPORE, A.G. ... et al, 2014. A bioartificial renal tubule device embedding human renal stem/progenitor cells. PLoS ONE, 9 (1), e87496.
Abstract: We present a bio-inspired renal microdevice that resembles the in vivo structure of a kidney proximal tubule. For the first time, a population of tubular adult renal stem/progenitor cells (ARPCs) was embedded into a microsystem to create a bioengineered renal tubule. These cells have both multipotent differentiation abilities and an extraordinary capacity for injured renal cell regeneration. Therefore, ARPCs may be considered a promising tool for promoting regenerative processes in the kidney to treat acute and chronic renal injury. Here ARPCs were grown to confluence and exposed to a laminar fluid shear stress into the chip, in order to induce a functional cell polarization. Exposing ARPCs to fluid shear stress in the chip led the aquaporin-2 transporter to localize at their apical region and the Na+K+ATPase pump at their basolateral portion, in contrast to statically cultured ARPCs. A recovery of urea and creatinine of (20±5)% and (13±5)%, respectively, was obtained by the device. The microengineered biochip here-proposed might be an innovative “lab-on-a-chip” platform to investigate in vitro ARPCs behaviour or to test drugs for therapeutic and toxicological responses.
Description: This is an open-access article distributed under the terms of the Creative Commons Attribution License CC-BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor: This study was supported by grants from “Regione Puglia” (Progetti strategici 44/09 BISIMANE and PS 144/06). Authors at the National Nanotechnology Laboratory of CNR-NANO also acknowledge the support of the Italian Ministry of University and Research through the FIRB Contract RBNE08BNL7 (MERIT Program).
Version: Published
DOI: 10.1371/journal.pone.0087496
URI: https://dspace.lboro.ac.uk/2134/17805
Publisher Link: http://dx.doi.org/10.1371/journal.pone.0087496
ISSN: 1932-6203
Appears in Collections:Published Articles (Materials)

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