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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/17808

Title: Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability
Authors: Moffa, Maria
Polini, Alessandro
Sciancalepore, Anna G.
Persano, Luana
Mele, Elisa
Gioia Passione, L.
Potente, Giovanni
Pisignano, Dario
Issue Date: 2013
Publisher: © Royal Society of Chemistry
Citation: MOFFA, M. ... et al, 2013. Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability. Soft Matter, 9 (23), pp. 5529 - 5539.
Abstract: The objective of this study is elucidating the mechanisms by which the wettability of nanofibrous electrospun mats varies in polymer blends, and highlighting how this can play a pivotal role in enhancing the viability of cultured microvascular endothelial cells (EC). A functional microvascular network is essential for supplying bioengineered tissues with oxygen and nutrients while removing metabolic wastes. An in vitro pre-vascularization strategy consists of seeding EC on scaffolds, which in turn promotes cells infiltration, adhesion and functionality. We use electrospun poly-L-lactic acid (PLLA) and gelatin (Gel) as prototype materials for realizing nanofibrous scaffolds as bioartificial architectures to improve the proliferation and the functionality of human microvascular ECs (HMEC-1). HMEC-1 seeded on electrospun scaffolds adhere, remain viable, proliferate and positively express the endothelial cell marker CD31 particularly on blend PLLA/Gel fibers, which exhibit wettability enhanced with respect to both the constituent polymers, and are therefore especially promising constructs for promoting the formation of functional endothelial tissue. The wettability characteristics of the blend polymer fibrous scaffolds are modeled and discussed. These results can be valuable for the future design of pre-vascularized scaffolds with enhanced wettability properties for functional tissue engineered implants, with ECs able to form in perspective an effectively functioning vasculature upon implantation.
Description: This article is closed access.
Sponsor: The authors acknowledge the support of the Italian Ministry of University and Research through the FIRB Contract RBNE08BNL7 (MERIT Program).
Version: Published
DOI: 10.1039/c3sm50328c
URI: https://dspace.lboro.ac.uk/2134/17808
Publisher Link: http://dx.doi.org/10.1039/c3sm50328c
ISSN: 1744-683X
Appears in Collections:Closed Access (Materials)

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