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Title: In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas
Authors: Olszewski, P.
Willett, Thomas C.
Theodosiou, Eirini
Thomas, Owen R.T.
Walsh, James L.
Issue Date: 2013
Publisher: © American Institute of Physics
Citation: OLSZEWSKI, P. ... et al, 2013. In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas. Applied Physics Letters, 102 (20), 204104.
Abstract: Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.
Description: Copyright 2013 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the authors and the American Institute of Physics. This article appeared in the journal, Applied Physics Letters, and may be found at: http://dx.doi.org/10.1063/1.4807391
Sponsor: This work is funded by the Biotechnology and Biological Sciences Research Council/Bioprocessing Research Industry Club (BRIC) programme (Grant No. BB/F004982/1), and the Engineering and Physical Sciences Research Council (Grant No. EP/J005894/1).
Version: Published
DOI: 10.1063/1.4807391
URI: https://dspace.lboro.ac.uk/2134/18130
Publisher Link: http://dx.doi.org/10.1063/1.4807391
ISSN: 0003-6951
Appears in Collections:Published Articles (Chemical Engineering)

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