+44 (0)1509 263171
Please use this identifier to cite or link to this item:
|Title: ||Multiplexed femtomolar quantitation of human cytokines in a fluoropolymer microcapillary film|
|Authors: ||Reis, Nuno M.|
Castanheira, Ana P.
Barbosa, Ana I.
Edwards, Alexander D.
|Issue Date: ||2015|
|Publisher: ||The Royal Society of Chemistry|
|Citation: ||REIS, N.M. ... et al, 2015. Multiplexed femtomolar quantitation of human cytokines in a fluoropolymer microcapillary film. Analyst, 140, pp.5609-5618|
|Abstract: ||Sensitive quantitation of multiple cytokines can provide important diagnostic information during
infection, inflammation and immunopathology. In this study sensitive immunoassay detection of human
cytokines IL-1β, IL-6, IL-12p70 and TNFα is shown for singleplex and multiplex formats using a novel
miniaturized ELISA platform. The platform uses a disposable plastic multi-syringe aspirator (MSA)
integrating 8 disposable fluoropolymer microfluidic test strips, each containing an array of ten 200 μm
mean i.d. microcapillaries coated with a set of monoclonal antibodies. Each MSA device thus performs 10
tests on 8 samples, delivering 80 measurements. Unprecedented levels of sensitivity were obtained with
the novel fluoropolymer microfluidic material and simple colorimetric detection in a flatbed scanner. The
limit of detection for singleplex detection ranged from 2.0 to 15.0 pg/ml, i.e. 35 and 713 femtomolar for
singleplex cytokine detection, and the intra- and inter-assay coefficient of variation (CV) remained within
10%. In addition, a triplex immunoassay was developed for measuring IL-1β, IL-12p70 and TNFα
simultaneously from a given sample in the pg/ml range. These assays permit high sensitivity measurement
with rapid <15 min assay or detection from undiluted blood serum. The portability, speed and low-cost of
this system are highly suited to point-of-care testing and field diagnostics applications.|
|Description: ||This is an Open Access article licensed under a Creative Commons Attribution 3.0 Unported Licence.|
|Sponsor: ||Ana P. Castanheira was sponsored by the ERASMUS program.|
|Publisher Link: ||http://dx.doi.org/10.1039/C5AN00238A|
|Appears in Collections:||Published Articles (Chemistry)|
Files associated with this item:
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.