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Title: Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale
Authors: Zhang, Xiaowei
Stettler, Matthieu
De Sanctis, Dario
Perrone, Marco
Parolini, Nicola
Discacciati, Marco
De Jesus, Maria
Hacker, David
Quarteroni, Alfio
Wurm, Florian M.
Keywords: Disposable bioreactor
Mammalian cell culture
Orbital shaking
Oxygen transfer rate
Scale-up
Shaken bioreactor
Issue Date: 2009
Publisher: © Springer-Verlag Berlin Heidelberg
Citation: ZHANG, X. ... et al, 2009. Use of orbital shaken disposable bioreactors for mammalian cell cultures from the milliliter-scale to the 1,000-liter scale. IN: Eibl, R. and Eibl, D. (eds). Disposable Reactors. Berlin: Springer Berlin Heidelberg, pp.33-53.
Series/Report no.: Advances in Biochemical Engineering / Biotechnology;115
Abstract: Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel mammalian cell culture bioreactor systems for the rapid production of biologicals that require mammalian protein processing. Recently, orbitally shaken bioreactors at scales from 50 mL to 1,000 L have been explored for the cultivation of mammalian cells and are considered to be attractive alternatives to conventional stirred-tank bioreactors because of increased flexibility and reduced costs. Adequate oxygen transfer capacity was maintained during the scale-up, and strategies to increase further oxygen transfer rates (OTR) were explored, while maintaining favorable mixing parameters and low-stress conditions for sensitive lipid membrane-enclosed cells. Investigations from process development to the engineering properties of shaken bioreactors are underway, but the feasibility of establishing a robust, standardized, and transferable technical platform for mammalian cell culture based on orbital shaking and disposable materials has been established with further optimizations and studies ongoing.
Description: This paper is closed access.
Version: Closed access
DOI: 10.1007/10_2008_18
URI: https://dspace.lboro.ac.uk/2134/18752
Publisher Link: http://dx.doi.org/10.1007/10_2008_18
ISBN: 9783642018725
9783642018718
ISSN: 0724-6145
Appears in Collections:Closed Access (Maths)

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