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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/18777

Title: Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently
Authors: Butler, Stephen J.
McMahon, Brian K.
Pal, Robert
Parker, David
Walton, James W.
Keywords: Cell imaging
Serum bicarbonate
Issue Date: 2013
Publisher: © Wiley-VCH Verlag GmbH & Co.
Citation: BUTLER, S.J. ... et al, 2013. Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently. Chemistry - A European Journal, 19 (29), pp. 9511 - 9517.
Abstract: A series of five europium(III) complexes has been prepared from heptadentate N5O2 ligands that possess a brightness of more than 10 mM−1 cm−1 in water, following excitation over the range λ=330–355 nm. Binding of several oxy anions has been assessed by emission spectral titrimetric analysis, with the binding of simple carboxylates, lactate and citrate involving a common ligation mode following displacement of the coordinated water. Selectivity for bicarbonate allows the rapid determination of this anion in human serum, with Kd=37 mM (295 K). The complexes are internalised quickly into mammalian cells and exhibit a mitochondrial localisation at early time points, migrating after a few hours to reveal a predominant lysosomal distribution. Herein, we report the synthesis and complexation behaviour of strongly emissive europium (III) complexes that bind oxy-anions in aqueous media with an affinity and selectivity profile that is distinctively different from previously studied systems.
Description: This article is closed access.
Sponsor: We thank the ERC (FCC 266804) and CISbio Bioassys (J.W.W.) for support.
Version: Published
DOI: 10.1002/chem.201301273
URI: https://dspace.lboro.ac.uk/2134/18777
Publisher Link: http://dx.doi.org/10.1002/chem.201301273
ISSN: 0947-6539
Appears in Collections:Closed Access (Chemistry)

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