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|Title: ||Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently|
|Authors: ||Butler, Stephen J.|
McMahon, Brian K.
Walton, James W.
|Keywords: ||Cell imaging|
|Issue Date: ||2013|
|Publisher: ||© Wiley-VCH Verlag GmbH & Co.|
|Citation: ||BUTLER, S.J. ... et al, 2013. Bright mono-aqua europium complexes based on triazacyclononane that bind anions reversibly and permeate cells efficiently. Chemistry - A European Journal, 19 (29), pp. 9511 - 9517.|
|Abstract: ||A series of five europium(III) complexes has been prepared from heptadentate N5O2 ligands that possess a brightness of more than 10 mM−1 cm−1 in water, following excitation over the range λ=330–355 nm. Binding of several oxy anions has been assessed by emission spectral titrimetric analysis, with the binding of simple carboxylates, lactate and citrate involving a common ligation mode following displacement of the coordinated water. Selectivity for bicarbonate allows the rapid determination of this anion in human serum, with Kd=37 mM (295 K). The complexes are internalised quickly into mammalian cells and exhibit a mitochondrial localisation at early time points, migrating after a few hours to reveal a predominant lysosomal distribution. Herein, we report the synthesis and complexation behaviour of strongly emissive europium (III) complexes that bind oxy-anions in aqueous media with an affinity and selectivity profile that is distinctively different from previously studied systems.|
|Description: ||This article is closed access.|
|Sponsor: ||We thank the ERC (FCC 266804) and CISbio Bioassys (J.W.W.) for support.|
|Publisher Link: ||http://dx.doi.org/10.1002/chem.201301273|
|Appears in Collections:||Closed Access (Chemistry)|
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