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|Title: ||Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms|
|Authors: ||Nienow, Alvin W.|
Hewitt, Christopher J.
Heathman, Thomas R.J.
Glyn, Veronica A.M.
Hanga, Mariana P.
Rafiq, Qasim A.
|Issue Date: ||2016|
|Publisher: ||© The Authors. Published by Elsevier B.V.|
|Citation: ||NIENOW, A.W. ...et al., 2016. Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms. Biochemical Engineering Journal, 108, pp. 24–29.|
|Abstract: ||In our recent work in different bioreactors up to 2.5 L in scale, we have successfully cultured hMSCs using
the minimum agitator speed required for complete microcarrier suspension, NJS. In addition, we also
reported a scaleable protocol for the detachment from microcarriers in spinner flasks of hMSCs from two
donors. The essence of the protocol is the use of a short period of intense agitation in the presence of
enzymes such that the cells are detached; but once detachment is achieved, the cells are smaller than
the Kolmogorov scale of turbulence and hence not damaged. Here, the same approach has been effective
for culture at NJS and detachment in-situ in 15 mL ambrTM bioreactors, 100 mL spinner flasks and 250 mL
Dasgip bioreactors. In these experiments, cells from four different donors were used along with two
types of microcarrier with and without surface coatings (two types), four different enzymes and three
different growth media (with and without serum), a total of 22 different combinations. In all cases after
detachment, the cells were shown to retain their desired quality attributes and were able to proliferate.
This agitation strategy with respect to culture and harvest therefore offers a sound basis for a wide range
of scales of operation.|
|Description: ||This is an open access article published by Elsevier under the CC BY license
|Sponsor: ||The authors would like to acknowledge the Engineering and Physical Sciences Research
Council (EPSRC), Lonza Cologne AG and FUJIFILM Diosynth Biotechnologies for funding this work.|
|Publisher Link: ||http://dx.doi.org/10.1016/j.bej.2015.08.003|
|Appears in Collections:||Published Articles (Chemical Engineering)|
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