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Aptamer based dispersion assay using tunable resistive pulse sensing (TRPS)

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posted on 2015-10-05, 13:37 authored by Emily R. Billinge, Mark PlattMark Platt
Aggregates of micron sized beads were formed by the binding of anti-thrombin aptamer to its complement. The addition of the thrombin protein target caused a concentration-dependant dispersion of these aggregates, and their number was measured by tunable resistive pulse sensing. The technique allowed the detection of thrombin down to sub picomolar concentrations, and an increase in sensitivity over previous assays on the same platform. The sensitivity of the assay is attributed to each thrombin protein disrupting multiple aggregates resulting in a signal amplification.

Funding

The work was supported by Loughborough University Chemistry Department (start-up fund) and the European Commission for Research (Nano4Bio FP7-PEOPLE- 2012-CIG-321836).

History

School

  • Science

Department

  • Chemistry

Published in

Analytical Methods: advancing methods and applications

Citation

BILLINGE, E.R. and PLATT, M., 2015. Aptamer based dispersion assay using tunable resistive pulse sensing (TRPS). Analytical Methods, 7(20), pp. 8534-8538.

Publisher

Royal Society of Chemistry

Version

  • VoR (Version of Record)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/

Publication date

2015

Notes

This is an Open Access article licensed under a Creative Commons Attribution 3.0 Unported Licence.

ISSN

1759-9679

Language

  • en

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