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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/18947

Title: Aptamer based dispersion assay using tunable resistive pulse sensing (TRPS)
Authors: Billinge, Emily R.
Platt, Mark
Issue Date: 2015
Publisher: Royal Society of Chemistry
Citation: BILLINGE, E.R. and PLATT, M., 2015. Aptamer based dispersion assay using tunable resistive pulse sensing (TRPS). Analytical Methods, 7(20), pp. 8534-8538.
Abstract: Aggregates of micron sized beads were formed by the binding of anti-thrombin aptamer to its complement. The addition of the thrombin protein target caused a concentration-dependant dispersion of these aggregates, and their number was measured by tunable resistive pulse sensing. The technique allowed the detection of thrombin down to sub picomolar concentrations, and an increase in sensitivity over previous assays on the same platform. The sensitivity of the assay is attributed to each thrombin protein disrupting multiple aggregates resulting in a signal amplification.
Description: This is an Open Access article licensed under a Creative Commons Attribution 3.0 Unported Licence.
Sponsor: The work was supported by Loughborough University Chemistry Department (start-up fund) and the European Commission for Research (Nano4Bio FP7-PEOPLE- 2012-CIG-321836).
Version: Published
DOI: 10.1039/C5AY01655J
URI: https://dspace.lboro.ac.uk/2134/18947
Publisher Link: http://dx.doi.org/10.1039/C5AY01655J
ISSN: 1759-9679
Appears in Collections:Published Articles (Chemistry)

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