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Title: Application of microneedle arrays for enhancement of transdermal permeation of Insulin: in vitro experiments, scaling analyses and numerical simulations
Authors: Leeladurga, V.
Chandra Teja, U.
Ashraf Sultana, S.K.
Sudeep, K.
Sai Sri Anusha, V
Han, Tao
Nalluri, Buchi N.
Das, Diganta Bhusan
Keywords: Histological studies
Insulin skin permeation
Microneedle arrays
Numerical simulations
Scaling analyses
Issue Date: 2016
Publisher: © American Association of Pharmaceutical Scientists. Published by Springer
Citation: LEELADURGA, V. ...et al., 2016. Application of microneedle arrays for enhancement of transdermal permeation of Insulin: in vitro experiments, scaling analyses and numerical simulations. AAPS PharmSciTech, 17 (4), pp. 915-922.
Abstract: The aim of this investigation is to study the effect of donor concentration and microneedle (MN) length on permeation of insulin and further evaluating the data using scaling analyses and numerical simulations. Histological evaluation of skin sections was carried to evaluate the skin disruption and depth of penetration by MNs. Scaling analyses was done using dimensionless parameters like concentration of drug (Ct/Cs), thickness (h/L) and surface area of the skin (Sa/L2). Simulation studies were carried out using MATLAB and COMSOL software to simulate the insulin permeation using histological sections of MN treated skin and experimental parameters like passive diffusion coefficient. A 1.6 fold increase in transdermal flux and 1.9 fold decrease in lag time values were observed with 1.5mm MN when compared with passive studies. Good correlation (R2>0.99) was observed between different parameters using scaling analyses. Also, the in vitro and simulated permeations profiles were found to be similar (f2≥50). Insulin permeation significantly increased with increase in donor concentration and MN length (p<0.05). The developed scaling correlations and numerical simulations were found to be accurate and would help researchers to predict the permeation of insulin with new dimensions of MN in optimizing insulin delivery. Overall, it can be inferred that the application of MNs can significantly enhance insulin permeation and may be an efficient alternative for injectable insulin therapy in humans.
Description: The final publication is available at Springer via http://dx.doi.org/10.1208/s12249-015-0416-8
Version: Accepted for publication
DOI: 10.1208/s12249-015-0416-8
URI: https://dspace.lboro.ac.uk/2134/19315
Publisher Link: http://dx.doi.org/10.1208/s12249-015-0416-8
ISSN: 1530-9932
Appears in Collections:Published Articles (Chemical Engineering)

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