SHAW, A. ...et al., 2015. A reference protocol for comparing the biocidal properties of gas plasma generating devices. Journal of Physics D: Applied Physics, 48(48).
The growing interest in the use of
non-thermal, atmospheric pressure gas plasmas for decontamination purposes has resulted in a multiplicity of plasma-
generating devices. There is currently no uni
versally approved method of comparing the
biocidal performance of such devices and in the work described here spores of the Gram
positive bacterium Bacillus subtilis (ATCC 6633) are proposed as a suitable reference
biological agent. In order to achieve consistency in the form in which the biological agent in question is presented to the plasma, a polycarbonate membrane loaded with a monolayer of spores is proposed. The advantages of the proposed protocol are evaluated by comparing inactivation tests in which an alternative microorganism (methicillin resistant Staphylococcus aureus
- MRSA) and the widely-used sample preparation technique of directly pipetting cell suspensions onto membranes are employed. In all cases, inactivation tests with either UV irradiation or plasma exposure were more reproducible when the proposed protocol was followed.
This paper is in closed access until 5th Nov 2016.
This work was supported by the UK Engineer
ing and Physical Sciences Research
Council (EPSRC), Loughborough University
and the University of Bologna.