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Title: A reference protocol for comparing the biocidal properties of gas plasma generating devices
Authors: Shaw, Alexander H.
Seri, P.
Borghi, Carlo A.
Shama, Gilbert
Iza, Felipe
Keywords: Bacillus subtilis spores
Gas plasma
UV irradiation
Bacteria-laden membranes
Issue Date: 2015
Publisher: © IOP Publishing
Citation: SHAW, A. ...et al., 2015. A reference protocol for comparing the biocidal properties of gas plasma generating devices. Journal of Physics D: Applied Physics, 48(48).
Abstract: The growing interest in the use of non-thermal, atmospheric pressure gas plasmas for decontamination purposes has resulted in a multiplicity of plasma- generating devices. There is currently no uni versally approved method of comparing the biocidal performance of such devices and in the work described here spores of the Gram positive bacterium Bacillus subtilis (ATCC 6633) are proposed as a suitable reference biological agent. In order to achieve consistency in the form in which the biological agent in question is presented to the plasma, a polycarbonate membrane loaded with a monolayer of spores is proposed. The advantages of the proposed protocol are evaluated by comparing inactivation tests in which an alternative microorganism (methicillin resistant Staphylococcus aureus - MRSA) and the widely-used sample preparation technique of directly pipetting cell suspensions onto membranes are employed. In all cases, inactivation tests with either UV irradiation or plasma exposure were more reproducible when the proposed protocol was followed.
Description: This is an author-created, un-copyedited version of an article accepted for publication in Journal of Physics D: Applied Physics. The publisher is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/0022-3727/48/48/484001.
Sponsor: This work was supported by the UK Engineering and Physical Sciences Research Council (EPSRC), Loughborough University and the University of Bologna.
Version: Accepted for publication
DOI: 10.1088/0022-3727/48/48/484001
URI: https://dspace.lboro.ac.uk/2134/19696
Publisher Link: http://dx.doi.org/10.1088/0022-3727/48/48/484001
ISSN: 0022-3727
Appears in Collections:Published Articles (Chemical Engineering)
Published Articles (Mechanical, Electrical and Manufacturing Engineering)

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