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Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila
journal contribution
posted on 2015-12-07, 10:27 authored by Marco Lucas, Carla Amaral, Ana Sampaio, Jose A. Peres, Albino A. DiasThis work looks for a better understanding of the biodegradation of xenobiotic azo dyes mediated by yeasts. During a screening program of
phenolic acid assimilating capacities it was found that a non-conventional ascomycetous yeast isolate, identified as Candida oleophila, efficiently
decolorizes agar plates supplemented with the commercial textile diazo dye Reactive Black 5. Aerobic batch cultures of C. oleophila could
completely decolorize up to 200 mg dye l−1, an ability not yet reported for this yeast species. Moreover, this performance has been achieved in just
24 h of incubation at 26 ◦C in the presence of as little as 5 g glucose l−1 and without visible signs of dye adsorption to yeast cells. It was found that
decolorization occurs during the exponential growth phase and neither laccase nor manganese-dependent peroxidase activities were detected in
the culture medium. As far as the decolorization mechanism is concerned, our results indirectly suggest the involvement of an azoreductase-like
activity in azo bonds cleavage.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Enzyme and Microbial TechnologyVolume
39Issue
1Pages
51 - 55Citation
LUCAS, M.S. ... et al, 2006. Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila. Enzyme and Microbial Technology, 39 (1), pp.51-55Publisher
© Elsevier IncVersion
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2006Notes
This paper is Closed Access.ISSN
0141-0229eISSN
1879-0909Publisher version
Language
- en