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|Title: ||Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila|
|Authors: ||Lucas, Marco S.|
Peres, Jose A.
Dias, Albino A.
|Issue Date: ||2006|
|Publisher: ||© Elsevier Inc|
|Citation: ||LUCAS, M.S. ... et al, 2006. Biodegradation of the diazo dye Reactive Black 5 by a wild isolate of Candida oleophila. Enzyme and Microbial Technology, 39 (1), pp.51-55|
|Abstract: ||This work looks for a better understanding of the biodegradation of xenobiotic azo dyes mediated by yeasts. During a screening program of
phenolic acid assimilating capacities it was found that a non-conventional ascomycetous yeast isolate, identified as Candida oleophila, efficiently
decolorizes agar plates supplemented with the commercial textile diazo dye Reactive Black 5. Aerobic batch cultures of C. oleophila could
completely decolorize up to 200 mg dye l−1, an ability not yet reported for this yeast species. Moreover, this performance has been achieved in just
24 h of incubation at 26 ◦C in the presence of as little as 5 g glucose l−1 and without visible signs of dye adsorption to yeast cells. It was found that
decolorization occurs during the exponential growth phase and neither laccase nor manganese-dependent peroxidase activities were detected in
the culture medium. As far as the decolorization mechanism is concerned, our results indirectly suggest the involvement of an azoreductase-like
activity in azo bonds cleavage.|
|Description: ||This paper is Closed Access.|
|Version: ||Closed access|
|Publisher Link: ||http://dx.doi.org/10.1016/j.enzmictec.2005.09.004|
|Appears in Collections:||Closed Access (Chemical Engineering)|
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