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Title: On-line coupling of gel electrophoresis and inductively coupled plasma-mass spectrometry
Authors: Haider, Syed R.
Sharp, Barry L.
Reid, Helen J.
Keywords: Agarose gel
Buffer contamination
Capillary electrophoresis (CE)
Elution buffer
High-performance liquid chromatography (HPLC)
Inductively coupled plasma-mass spectrometry (ICP-MS)
On-line coupling
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
Issue Date: 2011
Publisher: © Elsevier
Citation: HAIDER, S.R., SHARP, B.L. and REID, H.J., 2011. On-line coupling of gel electrophoresis and inductively coupled plasma-mass spectrometry. TrAC-Trends in Analytical Chemistry, 30(11), pp. 1793-1808.
Abstract: The on-line coupling of gel electrophoresis with inductively coupled plasma-mass spectrometry (GE-ICP-MS) is a powerful tool for simultaneous separation, detection and quantification of bio-molecules, and has been applied to the determination of phosphorus in DNA, phosphoproteins, and phosphopeptides, gold in nano-particles, iron in metalloproteins, and iodine in aerosols, and cisplatin-oligonucleotide interactions. However, since the first report in 2005, relatively few papers have been published, perhaps reflecting the lack of familiarity with the benefits of this promising methodology. So, here for the first time, we critically review the applications of GE-ICP-MS, and explore the advantages and the limitations of the technique for various applications. Such scrutiny may be useful in not only the development of the technique but also highlighting its potential in proteomics, genomics and metallomics.
Description: This paper was accepted for publication in the journal TrAC-Trends in Analytical Chemistry and the definitive published version is available at http://dx.doi.org/10.1016/j.trac.2011.04.025
Version: Accepted for publication
DOI: 10.1016/j.trac.2011.04.025
URI: https://dspace.lboro.ac.uk/2134/19972
Publisher Link: http://dx.doi.org/10.1016/j.trac.2011.04.025
ISSN: 0165-9936
Appears in Collections:Published Articles (Chemistry)

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