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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/20179

Title: Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing
Authors: Blundell, Emma L.C.J.
Vogel, Robert
Platt, Mark
Issue Date: 2016
Publisher: © American Chemical Society
Citation: BLUNDELL, E.L.C.J., VOGEL, R. and PLATT, M., 2016. Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing. Langmuir, 32(4), pp.1082-1090
Abstract: Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterized as they traverse pores. Previous work using RPS has shown that the size, concentration and zeta potential of the analyte can be measured. Here we use tunable resistive pulse sensing (TRPS) which utilizes a tunable pore to monitor the translocation times of nanoparticles with DNA modified surfaces. We start by demonstrating that the translocation times of particles can be used to infer the zeta potential of known standards and then apply the method to measure the change in zeta potential of DNA modified particles. By measuring the translocation times of DNA modified nanoparticles as a function of packing density, length, structure, and hybridization time, we observe a clear difference in zeta potential using both mean values and population distributions as a function of the DNA structure. We demonstrate the ability to resolve the signals for ssDNA, dsDNA, small changes in base length for nucleotides between 15 and 40 bases long, and even the discrimination between partial and fully complementary target sequences. Such a method has potential and applications in sensors for the monitoring of nanoparticles in both medical and environmental samples.
Description: This paper is closed access until January 12th 2017.
Sponsor: European Commission for Research (PCIG11-GA-2012-321836 Nano4Bio), Loughborough University Chemistry Department (Start-up fund) and by the National Institute for Health Research (NIHR) Diet, Lifestyle & Physical Activity Biomedical Research Unit based at University Hospitals of Leicester and Loughborough University.
Version: Accepted for publication
DOI: 10.1021/acs.langmuir.5b03024
URI: https://dspace.lboro.ac.uk/2134/20179
Publisher Link: http://dx.doi.org/10.1021/acs.langmuir.5b03024
ISSN: 1520-5827
Appears in Collections:Closed Access (Chemistry)

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