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|Title: ||Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate|
|Authors: ||Heathman, Thomas R.J.|
Rafiq, Qasim A.
Nienow, Alvin W.
Hewitt, Christopher J.
|Keywords: ||Human platelet lysate|
Mesenchymal stromal cell
|Issue Date: ||2016|
|Citation: ||HEATHMAN, T.R.J. ... et al., 2016. Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate. Cytotherapy, 18(4), pp.523-535.|
|Abstract: ||Background: The selection of medium and associated reagents for human mesenchymal
stromal cell (hMSC) culture forms an integral part of manufacturing process development and
must be suitable for multiple process scales and expansion technologies.
Methods: In this work, we have expanded BM-hMSCs in fetal bovine serum (FBS)- and human
platelet lysate (HPL)-containing media in both a monolayer and a suspension-based
Results: The introduction of HPL into the monolayer process increased the BM-hMSC growth
rate at the first experimental passage by 0.049 day-1
and 0.127 day-1
for the two BM-hMSC
donors compared to the FBS-based monolayer process. This increase in growth rate in HPLcontaining
medium was associated with an increase in the inter-donor consistency, with an
inter-donor range of 0.406 cumulative population doublings after 18 days compared with 2.013
in FBS-containing medium. Identity and quality characteristics of the BM-hMSCs are also
comparable between conditions, in terms of colony-forming potential, osteogenic potential and
expression of key genes during monolayer and post-harvest from microcarrier expansion. BMhMSCs
cultured on microcarriers in HPL-containing medium demonstrated a reduction in the
initial lag phase for both BM-hMSC donors and an increased BM-hMSC yield following six
days of culture to 1.20 ± 0.17 x 105
and 1.02 ± 0.005 x 105
compared with 0.79 ±
0.05 x 105
and 0.36 ± 0.04 x 105
in FBS-containing medium.
Conclusions: This study has demonstrated that HPL, in comparison with FBS-containing
medium, delivers increased growth and comparability across two BM-hMSC donors between
monolayer and microcarrier culture, which will have key implications for process transfer
|Description: ||This is an Open Access Article. It is published by Elsevier under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/|
|Sponsor: ||This study has been funded by the Engineering and Physical Sciences Research Council
(EPSRC) and FUJIFILM Diosynth Biotechnologies.|
|Publisher Link: ||http://dx.doi.org/10.1016/j.jcyt.2016.01.007|
|Appears in Collections:||Published Articles (Chemical Engineering)|
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