Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Loughborough University Institutional Repository

Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/20586

Title: Rapid determination of N-Methylpyrrolidine in Cefepime by combining direct infusion electrospray ionisation-time-of-flight mass spectrometry with field asymmetric waveform ion mobility spectrometry
Authors: Smith, Robert W.
Cox, Lisa B.
Yudin, Aswandi
Reynolds, James C.
Powell, Mark
Creaser, Colin S.
Keywords: Drug impurity
FAIMS
Electrospray mass spectrometry
N-methyl pyrrolidine
Cefepime
Issue Date: 2015
Publisher: Royal Society of Chemistry
Citation: SMITH, R.W. ...et al., 2015. Rapid determination of N-Methylpyrrolidine in Cefepime by combining direct infusion electrospray ionisation-time-of-flight mass spectrometry with field asymmetric waveform ion mobility spectrometry. Analytical Methods, 7, pp. 34-39.
Abstract: The determination of N-methyl pyrrolidine, a potential impurity in the cephalosporin antibiotic cefepime, by direct infusion ESI combined with field asymmetric waveform ion mobility spectrometry-mass spectrometry (ESI-FAIMS-MS) is demonstrated. The addition of a chip-based FAIMS separation prior to detection by time-of-flight mass spectrometry enables selective transmission of NMP in the presence of cefepime without interference from NMP formed by CID in the mass spectrometer interface. The limits of detection and quantification of NMP in cefepime were 0.011% (w/w) and 0.036% (w/w) NMP in cefepime respectively, well below the 0.3% (w/w) threshold concentration for NMP in cefepime. The % relative standard deviation was 3.9% with linearity for standard additions in the range 0.005 – 0.5 μg/ml NMP. Novel Aspect (ToC) FAIMS separation prior to mass spectrometry enables selective transmission of NMP in cefepime without interference from NMP formed by in-source CID.
Description: This paper was accepted for publication in the journal Analytical Methods and the definitive published version is available at: http://dx.doi.org/10.1039/c4ay02026j
Version: Accepted for publication
DOI: 10.1039/c4ay02026j
URI: https://dspace.lboro.ac.uk/2134/20586
Publisher Link: http://dx.doi.org/10.1039/c4ay02026j
ISSN: 1759-9679
Appears in Collections:Published Articles (Chemistry)

Files associated with this item:

File Description SizeFormat
Smith et al_NMP in cefepime_Analytical Methods 2015_accepted manuscript.pdfAccepted version515.57 kBAdobe PDFView/Open

 

SFX Query

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.