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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/20991

Title: A dielectrophoretic method of discrimination between normal oral epithelium, and oral and oropharyngeal cancer in a clinical setting
Authors: Graham, K.A.
Mulhall, H.J.
Labeed, F.H.
Lewis, Mark P.
Hoettges, K.F.
Kalavrezos, N.
McCaul, J.
Liew, C.
Porter, S.
Fedele, S.
Hughes, M.P.
Keywords: Oropharyngeal carcinoma
Issue Date: 2015
Publisher: © RSC
Citation: GRAHAM, K. ... et al., 2015. A dielectrophoretic method of discrimination between normal oral epithelium, and oral and oropharyngeal cancer in a clinical setting. Analyst, 140 (15), pp.5198-5204.
Abstract: Despite the accessibility of the oral cavity to clinical examination, delays in diagnosis of oral and oropharyngeal carcinoma (OOPC) are observed in a large majority of patients, with negative impact on prognosis. Diagnostic aids might help detection and improve early diagnosis, but there remains little robust evidence supporting the use of any particular diagnostic technology at the moment. The aim of the present feasibility first-in-human study was to evaluate the preliminary diagnostic validity of a novel technology platform based on dielectrophoresis (DEP). DEP does not require labeling with antibodies or stains and it is an ideal tool for rapid analysis of cell properties. Cells from OOPC/dysplasia tissue and healthy oral mucosa were collected from 57 study participants via minimally-invasive brush biopsies and tested with a prototype DEP platform using median membrane midpoint frequency as main analysis parameter. Results indicate that the current DEP platform can discriminate between brush biopsy samples from cancerous and healthy oral tissue with a diagnostic sensitivity of 81.6% and a specificity of 81.0%. The present ex vivo results support the potential application of DEP testing for identification of OOPC. This result indicates that DEP has the potential to be developed into a low-cost, rapid platform as an assistive tool for the early identification of oral cancer in primary care; given the rapid, minimally-invasive and non-expensive nature of the test, dielectric characterization represents a promising platform for cost-effective early cancer detection.
Sponsor: We would like to thank the National Institute for Health Research-NIHR for funding this work through Research for Innovation, Speculation and Creativity Program RC-PG-0407-10123 and Invention for Innovation Program ES-1010-10163, as well as the Finance South East Collaboration Fund. UCL/UCLH received a proportion of funding from the Department of Health’s NIHR Biomedical Research Centre funding scheme. KAG was funded by a PhD studentship from Labtech International.
Version: Published
DOI: 10.1039/c5an00796h
URI: https://dspace.lboro.ac.uk/2134/20991
Publisher Link: http://dx.doi.org/10.1039/c5an00796h
ISSN: 0003-2654
Appears in Collections:Published Articles (Sport, Exercise and Health Sciences)

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