+44 (0)1509 263171
Please use this identifier to cite or link to this item:
|Title: ||Protein detection using tunable pores: resistive pulses and current rectification|
|Authors: ||Blundell, Emma L.C.J.|
Mayne, Laura J.
Christie, Steven D.R.
|Issue Date: ||2016|
|Publisher: ||© Royal Society of Chemistry|
|Citation: ||BLUNDELL, E. ... et al, 2016. Protein detection using tunable pores: resistive pulses and current rectification. Faraday Discussions, 193 (Nov), pp.487-505|
|Abstract: ||We present the first comparison between assays that use resistive pulses or rectification ratios on a tunable pore platform. We compare their ability quantify the cancer biomarker Vascular Endothelial Growth Factor (VEGF). The first assay measures the electrophoretic mobility of aptamer modified nanoparticles as they traverse the pore. By controlling the aptamer loading on the particles surface, and measuring the speed of each translocation event we are able to observe a change in velocity as low as 18 pM. A second non-particle assay exploits the current rectification properties of conical pores. We report the first use of Layer-by-Layer, (LbL) assembly of polyelectrolytes onto the surface of the polyurethane pore. The current rectification ratios demonstrate the presence of the polymers, producing pH and ionic strength dependent currents. The LbL allows the facile immobilisation of DNA aptamers onto the pore allowing a specific dose response to VEGF. Monitoring changes to the current rectification allows for a rapid detection of VEGF 5 pM. Each assay format offers advantages in preparation but comparable sensitivities|
|Description: ||This paper was accepted for publication in the journal Faraday Discussions and the definitive published version is available at http://dx.doi.org/10.1039/C6FD00072J|
|Sponsor: ||The work was supported by the European Commission for Research
(PCIG11-GA-2012-321836 Nano4Bio), Loughborough University
Chemistry Department (Start-up fund).|
|Version: ||Accepted for publication|
|Publisher Link: ||http://dx.doi.org/10.1039/C6FD00072J|
|Appears in Collections:||Published Articles (Chemistry)|
Files associated with this item:
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.