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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/21222

Title: Protein detection using tunable pores: resistive pulses and current rectification
Authors: Blundell, Emma L.C.J.
Mayne, Laura J.
Lickorish, Michael
Platt, Mark
Christie, Steven D.R.
Issue Date: 2016
Publisher: © Royal Society of Chemistry
Citation: BLUNDELL, E. ... et al, 2016. Protein detection using tunable pores: resistive pulses and current rectification. Faraday Discussions, DOI: 10.1039/C6FD00072J
Abstract: We present the first comparison between assays that use resistive pulses or rectification ratios on a tunable pore platform. We compare their ability quantify the cancer biomarker Vascular Endothelial Growth Factor (VEGF). The first assay measures the electrophoretic mobility of aptamer modified nanoparticles as they traverse the pore. By controlling the aptamer loading on the particles surface, and measuring the speed of each translocation event we are able to observe a change in velocity as low as 18 pM. A second non-particle assay exploits the current rectification properties of conical pores. We report the first use of Layer-by-Layer, (LbL) assembly of polyelectrolytes onto the surface of the polyurethane pore. The current rectification ratios demonstrate the presence of the polymers, producing pH and ionic strength dependent currents. The LbL allows the facile immobilisation of DNA aptamers onto the pore allowing a specific dose response to VEGF. Monitoring changes to the current rectification allows for a rapid detection of VEGF 5 pM. Each assay format offers advantages in preparation but comparable sensitivities
Description: This paper is embargoed until April 2017.
Sponsor: The work was supported by the European Commission for Research (PCIG11-GA-2012-321836 Nano4Bio), Loughborough University Chemistry Department (Start-up fund).
Version: Accepted for publication
DOI: 10.1039/C6FD00072J
URI: https://dspace.lboro.ac.uk/2134/21222
Publisher Link: http://dx.doi.org/10.1039/C6FD00072J
ISSN: 1364-5498
Appears in Collections:Closed Access (Chemistry)

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