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Title: Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals
Authors: Taylor, Conor W.
Ingham, Stephen A.
Hunt, Julie E.A.
Martin, Neil R.W.
Pringle, Jamie S.M.
Ferguson, Richard A.
Keywords: Angiogenesis
HIF-1α
High-intensity training
Mitochondrial biogenesis
Sprint interval exercise
Issue Date: 2016
Publisher: Springer / © The Authors
Citation: TAYLOR, C.W. ... et al, 2016. Exercise duration-matched interval and continuous sprint cycling induce similar increases in AMPK phosphorylation, PGC-1α and VEGF mRNA expression in trained individuals. European Journal of Applied Physiology, 116 (8), pp. 1445-1454.
Abstract: Purpose: The effects of low-volume interval and continuous ‘all-out’ cycling, matched for total exercise duration, on mitochondrial and angiogenic cell signalling was investigated in trained individuals. Methods: In a repeated measures design, 8 trained males ((Formula presented.), 57 ± 7 ml kg−1 min−1) performed two cycling exercise protocols; interval (INT, 4 × 30 s maximal sprints interspersed by 4 min passive recovery) or continuous (CON, 2 min continuous maximal sprint). Muscle biopsies were obtained before, immediately after and 3 h post-exercise. Results: Total work was 53 % greater (P = 0.01) in INT compared to CON (71.2 ± 7.3 vs. 46.3 ± 2.7 kJ, respectively). Phosphorylation of AMPKThr172 increased by a similar magnitude (P = 0.347) immediately post INT and CON (1.6 ± 0.2 and 1.3 ± 0.3 fold, respectively; P = 0.011), before returning to resting values at 3 h post-exercise. mRNA expression of PGC-1α (7.1 ± 2.1 vs. 5.5 ± 1.8 fold; P = 0.007), VEGF (3.5 ± 1.2 vs. 4.3 ± 1.8 fold; P = 0.02) and HIF-1α (2.0 ± 0.5 vs. 1.5 ± 0.3 fold; P = 0.04) increased at 3 h post-exercise in response to INT and CON, respectively; the magnitude of which were not different between protocols. Conclusions: Despite differences in total work done, low-volume INT and CON ‘all-out’ cycling, matched for exercise duration, provides a similar stimulus for the induction of mitochondrial and angiogenic cell signalling pathways in trained skeletal muscle.
Description: This is an open access article published by Springer and distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), https://creativecommons.org/licenses/by/4.0/
Version: Published
DOI: 10.1007/s00421-016-3402-2
URI: https://dspace.lboro.ac.uk/2134/21909
Publisher Link: http://dx.doi.org/10.1007/s00421-016-3402-2
ISSN: 1439-6319
Appears in Collections:Published Articles (Sport, Exercise and Health Sciences)

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