SARPARA, G. ... et al., 1999. A new long-wavelength fluorigenic substrate for alkaline phosphatase: synthesis and characterisation. Analytical Communications, 36, pp.19-20.
Naphthofluorescein diphosphate has been synthesised from
the parent dye, and shown to be an attractive longwavelength
alternative to other fluorigenic substrates for the
determination of alkaline phosphatase. Its application to the
determination of theophylline, an inhibitor of this enzyme,
has been demonstrated. The optimum excitation wavelength
of the hydrolysis product naphthofluorescein has been found
to depend on the presence of additives such as cyclodextrins
and (3-[3-cholamidopropyl]-dimethylamino)-1-propane sulfonate
(CHAPS): such effects can be used to raise the
excitation wavelength to match the output of a 635 nm diode
laser in a simple and sensitive fluorescence detector.
G.H.S. thanks the Government of Pakistan for financial