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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/22230

Title: A compact long-wavelength fluorescence detection system and its application to flow injection immunoassay
Authors: Hu, Sijung
French, Martin T.
Palmer, Derek A.
Evans, Mark
Zhou, Sue M.
Sarpara, G. Hussain
Miller, James N.
Keywords: Long-wavelength fluorescence
Cyanine dyes
Naphthofluorescein
Immunoassay
α-Interferon
Issue Date: 2002
Publisher: © Elsevier
Citation: HU, S. ... et al., 2002. A compact long-wavelength fluorescence detection system and its application to flow injection immunoassay. Analytica Chimica Acta, 454 (1), pp.31-35.
Abstract: A compact and robust long-wavelength fluorescence detection system (LWFDS) has been constructed using a pulsed diode laser as the light source and a photodiode detector. The detection limits for naphthofluorescein (NF) and the cyanine dyes Cy5 and Cy5.5 at pH 8.8, determined using the LWFDS, were 1.0 × 10-10, 2.3 × 10-12 and 6.2 × 10-11M, respectively. These values compared favourably with data from a commercial research grade fluorescence spectrometer. The LWFDS instrument was used as the detector for a competitive heterogeneous flow injection immunoassay for α-interferon with Cy5 as the label. A calibration graph usable over the range 0.5-10 μg ml-1 α-interferon was obtained. The instrument demonstrated its potential for rapid ( <200s per sample) multi-analyte assays and for field monitoring.
Description: Closed access.
Sponsor: S.J.H. acknowledges financial support from Loughborough University and from Kalibrant Limited. We also thank Glaxo-Wellcome for gifts of α-interferon and its antibodies.
Version: Published
DOI: 10.1016/S0003-2670(01)01468-4
URI: https://dspace.lboro.ac.uk/2134/22230
Publisher Link: http://dx.doi.org/10.1016/S0003-2670(01)01468-4
ISSN: 0003-2670
Appears in Collections:Closed Access (Mechanical, Electrical and Manufacturing Engineering)

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