+44 (0)1509 263171
Please use this identifier to cite or link to this item:
|Title: ||A compact long-wavelength fluorescence detection system and its application to flow injection immunoassay|
|Authors: ||Hu, Sijung|
French, Martin T.
Palmer, Derek A.
Zhou, Sue M.
Sarpara, G. Hussain
Miller, James N.
|Keywords: ||Long-wavelength fluorescence|
|Issue Date: ||2002|
|Publisher: ||© Elsevier|
|Citation: ||HU, S. ... et al., 2002. A compact long-wavelength fluorescence detection system and its application to flow injection immunoassay. Analytica Chimica Acta, 454 (1), pp.31-35.|
|Abstract: ||A compact and robust long-wavelength fluorescence detection system (LWFDS) has been constructed using a pulsed diode laser as the light source and a photodiode detector. The detection limits for naphthofluorescein (NF) and the cyanine dyes Cy5 and Cy5.5 at pH 8.8, determined using the LWFDS, were 1.0 × 10-10, 2.3 × 10-12 and 6.2 × 10-11M, respectively. These values compared favourably with data from a commercial research grade fluorescence spectrometer. The LWFDS instrument was used as the detector for a competitive heterogeneous flow injection immunoassay for α-interferon with Cy5 as the label. A calibration graph usable over the range 0.5-10 μg ml-1 α-interferon was obtained. The instrument demonstrated its potential for rapid ( <200s per sample) multi-analyte assays and for field monitoring.|
|Description: ||Closed access.|
|Sponsor: ||S.J.H. acknowledges financial support from Loughborough University and from Kalibrant Limited. We also thank Glaxo-Wellcome for gifts of α-interferon and its antibodies.|
|Publisher Link: ||http://dx.doi.org/10.1016/S0003-2670(01)01468-4|
|Appears in Collections:||Closed Access (Mechanical, Electrical and Manufacturing Engineering)|
Files associated with this item:
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.