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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/22569

Title: Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier for silver sulfadiazine nanocrystals
Authors: Gao, Lei
Gan, Hui
Meng, Zhiyun
Gu, Ruolan
Wu, Zhuona
Zhu, Xiaoxia
Sun, Wenzhong
Li, Jian
Zheng, Ying
Sun, Tao
Dou, Guifang
Keywords: Silver Sulfadiazine
Wound healing
Issue Date: 2016
Publisher: © Elsevier
Citation: GAO, L. ...et al., 2016. Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier for silver sulfadiazine nanocrystals. Colloids and Surfaces B: Biointerfaces, 148, pp 343–353.
Abstract: In the present study genipin crosslinked chitosan (CHI) hydrogels, which had been constructed and reported in our previous studies (Lei Gao, et al. Colloids Surf. B Biointerfaces. 2014, 117: 398), were further evaluated for their advantage as a carrier for silver sulfadiazine (AgSD) nanocrystal systems. Firstly, AgSD nanocrystals with a mean particle size of 289 nm were prepared by wet milling method and encapsulated into genipin crosslinked CHI hydrogels. AgSD nanocrystals displayed a uniform distribution and very good physical stability in the hydrogel network. Swelling-dependent release pattern was found for AgSD nanocrystals from hydrogels and the release profile could be well fitted with Peppas equation. When AgSD nanocrystals were encapsulated in hydrogels their fibroblast cytotoxicity decreased markedly, and their antibacterial effects against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were still comparable to unencapsulated AgSD nanocrystals. In vivo evaluation in excision and burn cutaneous wound models in mice showed that AgSD nanocrystal hydrogels markedly decreased the expression of inflammatory cytokine IL-6, but increased the levels of growth factors VEGF-A and TGF-β1. Histopathologically, the wounds treated by hydrogels containing AgSD nanocrystals showed the best healing state compared with commercial AgSD cream, hydrogels containing AgSD bulk powders and blank hydrogels. The wounds treated by AgSD nanocrystal hydrogels were dominated by marked fibroblast proliferation, new blood vessels and thick regenerated epithelial layer. Sirius Red staining assay indicated that AgSD nanocrystal hydrogels resulted in more collagen deposition characterized by a large proportion of type I fibers. Our study suggested that genipin-crosslinked CHI hydrogel was a potential carrier for local antibacterial nanomedicines.
Description: This paper is in closed access until 11th June 2017.
Sponsor: This work was founded by the Newton Research Programme Foundation titled “ Investigation of host tissue integration using closely integrated experimental and computational approaches approved by the Royal Academy of Engineering, the project number is NRCP/1415/54.
Version: Accepted for publication
DOI: 10.1016/j.colsurfb.2016.06.016
URI: https://dspace.lboro.ac.uk/2134/22569
Publisher Link: http://dx.doi.org/10.1016/j.colsurfb.2016.06.016
ISSN: 0927-7765
Appears in Collections:Closed Access (Chemical Engineering)

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