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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/23099

Title: A tunable nanopore sensor for the detection of metal ions using translocation velocity and biphasic pulses
Authors: Mayne, Laura J.
Christie, Steven D.R.
Platt, Mark
Keywords: Nanopore
Tunable resistive pulse sensing
Sensor
Biphasic pulses
Issue Date: 2016
Publisher: Royal Society of Chemistry
Citation: MAYNE, L.J., CHRISTIE, S.D.R. and PLATT, M., 2016. A tunable nanopore sensor for the detection of metal ions using translocation velocity and biphasic pulses. Nanoscale, doi: 10.1039/C6NR07224K
Abstract: A Tunable Resistive Pulse Sensor utilising a Polyurethane nanopore, has been used to characterise nanoparticles as they traverse the pore opening. Here we demonstrate that the translocation speed, conductive and resistive pulse magnitude, can be used to infer the surface charge of a nanoparticle, and act as a specific transduction signal for the binding of metal ions to ligands on the particles surface. Surfaces of silica nanoparticles were modified with a ligand to demonstrate the concept, and used to extract copper (II) ions (Cu2+) from solution. By tuning the pH and ionic strength of the solution, a biphasic pulse, a conductive followed by a resistive pulse is recorded. Biphasic pulses are becoming a powerful way to characterise materials, and provide an insight into the translocation mechanism, and here we present their first use to detect the presence of metal ions in solution. We demonstrate how combinations of translocation speed and/ or biphasic pulse behaviour are used to detect Cu2+ with quantitative responses across a range of pH and ionic strengths. Using a generic ligand this assay allows a clear signal for Cu2+ as low as 1 ppm with short 5 minute incubation time, and capable of measuring 10 ppm Cu2+ in the presence of 5 other ions. The method has potential for monitoring heavy metals in biological and environmental samples.
Description: This paper is closed access until 27th October 2017.
Version: Accepted for publication
DOI: 10.1039/C6NR07224K
URI: https://dspace.lboro.ac.uk/2134/23099
Publisher Link: http://dx.doi.org/10.1039/C6NR07224K
ISSN: 2040-3372
Appears in Collections:Closed Access (Chemistry)

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