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Title: An overview of current techniques for ocular toxicity testing
Authors: Wilson, Samantha L.
Ahearne, Mark
Hopkinson, Andrew
Keywords: Corneal equivalents
Cytotoxicity
Draize
In vitro alternatives
Organotypic models
Regulation
Validation
Issue Date: 2014
Publisher: Elsevier © The Authors
Citation: WILSON, S.L., AHEARNE, M. and HOPKINSON, A., 2014. An overview of current techniques for ocular toxicity testing. Toxicology, 327, pp. 32-46.
Abstract: Given the hazardous nature of many materials and substances, ocular toxicity testing is required to evaluate the dangers associated with these substances after their exposure to the eye. Historically, animal tests such as the Draize test were exclusively used to determine the level of ocular toxicity by applying a test substance to a live rabbit’s eye and evaluating the biological response. In recent years, legislation in many developed countries has been introduced to try to reduce animal testing and promote alternative techniques. These techniques include ex vivo tests on deceased animal tissue, computational models that use algorithms to apply existing data to new chemicals and in vitro assays based on two dimensional (2D) and three dimensional (3D) cell culture models. Here we provide a comprehensive overview of the latest advances in ocular toxicity testing techniques, and discuss the regulatory framework used to evaluate their suitability.
Description: This is an Open Access article. It is published by Elsevier under the Creative Commons Attribution 3.0 Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/3.0/
Sponsor: Funding from EPSRC Engineering, Tissue Engineering and Regenerative Medicine Fellowship (E-TERM, Grant number: EP/ 1017801/1) and the University of Nottingham HERMES Fellowship (Grant number: 13b/I9)
Version: Published
DOI: 10.1016/j.tox.2014.11.003
URI: https://dspace.lboro.ac.uk/2134/23211
Publisher Link: http://dx.doi.org/10.1016/j.tox.2014.11.003
ISSN: 0300-483X
Appears in Collections:Published Articles (Mechanical, Electrical and Manufacturing Engineering)

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