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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/23463

Title: Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification
Authors: Morelli, Serena
Holdich, R.G.
Dragosavac, Marijana M.
Keywords: Dispersion cell membrane emulsification
Cell encapsulation
W/O emulsions
Eudragit S100 coating
Intestine-colon targeted release
Issue Date: 2017
Publisher: © Elsevier
Citation: MORELLI, S., HOLDICH, R. and DRAGOSAVAC, M., 2017. Microparticles for cell encapsulation and colonic delivery produced by membrane emulsification. Journal of Membrane Science, 524, pp.377-388.
Abstract: Membrane Emulsification was used to encapsulate yeast cells and form microparticles. W/O emulsions were produced using a Dispersion Cell; the aqueous phase consisted of gelatin/chitosan, or pure gelatin solution, containing yeast cells, the continuous phase was 2 wt% of SPAN 80 in kerosene. Varying the dispersed phase flux (from 70 to 350 L h- m-2) and the shear stress (from 17 to 1 Pa) applied on the membrane surface droplet sizes of between 60 and 340 µm were produced, with a coefficient of variation of 17% under the best operating conditions. The liquid drops were loaded with increasing amount of yeast (3.14×107 to 3.14×108 cells/mL). The stability and uniformity of the emulsions was independent of the cell concentration. PTFE coated hydrophobic membrane produced smaller W/O drops compared to FAS coated membranes. The liquid polymeric droplets were solidified in solid particles using thermal gelation and/or ionic crosslinking, obtaining yeast encapsulated particles sized ~100 µm. The pH sensitive polymer, Eudragit S100, was used as a coating to create gastro resistant particles suitable for intestinal-colonic targeted release. Viability of the released yeast cells was demonstrated using fluorescence probes and checking cell glucose metabolism with time.
Description: This paper was accepted for publication in the journal Journal of Membrane Science and the definitive published version is available at http://dx.doi.org/10.1016/j.memsci.2016.11.058.
Version: Accepted for publication
DOI: 10.1016/j.memsci.2016.11.058
URI: https://dspace.lboro.ac.uk/2134/23463
Publisher Link: http://dx.doi.org/10.1016/j.memsci.2016.11.058
ISSN: 0376-7388
Appears in Collections:Published Articles (Chemical Engineering)

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