Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Loughborough University Institutional Repository

Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/23649

Title: Engineering considerations on the use of liquid/liquid two phase systems as a cell culture platform
Authors: Murasiewicz, Halina
Nienow, Alvin W.
Hanga, Mariana P.
Coopman, Karen
Hewitt, Christopher J.
Pacek, Andrzej W.
Keywords: Liquid microcarriers
Cell culture
Emulsification
Perfluorocarbon
Bioreactors
Dispersion
Issue Date: 2016
Publisher: © John Wiley & Sons
Citation: MURASIEWICZ, H. ... et al., 2016. Engineering considerations on the use of liquid/liquid two phase systems as a cell culture platform. Journal of chemical technology and biotechnology, DOI: 10.1002/jctb.5166.
Abstract: BACKGROUND. Application of perfluorocarbon based liquid/liquid two phase systems for cell culture expansion has been investigated at small scale for more than 30 years and it has been established that such systems are able to support the survival of a variety of cell lines. Application of drops in liquid/liquid dispersions as temporary microcarriers is an exciting prospect as it enables adherent cells to be grown in stirred bioreactors, without the need to use enzymatic dissociation methods to harvest the cells. RESULTS. Two aspects of scaling up of perfluorocarbon/cell culture medium dispersions were investigated: (i) the effect of processing conditions on drop size/interfacial area and (ii) the kinetics of separation of a stagnant dispersion. The processing conditions to produce the stable “liquid microcarriers” with the average drop size between 150 - 220 µm have been established. Separation of dispersion into two continuous systems requires complete removal of proteins from the perfluorocarbon/cell culture media interface. CONCLUSIONS. The correlation relating average drop size to the energy input and physical properties of both phases was developed and the method of separation of stable perfluorocarbon/cell culture medium dispersion was established. As the perfluorocarbon does not deteriorate during cell expansion and subsequent separation followed by sterilization, it could be re-used, making application of such systems at a large scale very attractive and economical.
Description: Closed access until 10/12/2017.
Sponsor: This work has been financially supported by BBSRC BRIC (research grant BB/K011066/1 and BB/K01099/1).
Version: Accepted for publication
DOI: 10.1002/jctb.5166
URI: https://dspace.lboro.ac.uk/2134/23649
Publisher Link: http://dx.doi.org/10.1002/jctb.5166
ISSN: 0268-2575
Appears in Collections:Closed Access (Chemical Engineering)

Files associated with this item:

File Description SizeFormat
23649.pdfAccepted version2.43 MBAdobe PDFView/Open

 

SFX Query

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.