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Title: Volatile organic compound markers of psychological stress in skin: a pilot study
Authors: Martin, Helen J.
Turner, Matthew A.
Bandelow, Stephan
Edwards, Louisa
Riazanskaia, Svetlana
Thomas, C.L. Paul
Keywords: Skin
Polydimethylsilicone sampling
Benzoic acid
N-decanoic acid
Thermal desorption GC-MS
Issue Date: 2016
Publisher: © IOP Publishing
Citation: MARTIN, H.J. ... et al, 2016. Volatile organic compound markers of psychological stress in skin: a pilot study. Journal of Breath Research, 10 (4), 046012.
Abstract: The forehead was studied as a possible sampling site for capturing changes in volatile organic compound (VOC) profiles associated with psychological-stress. Skin-VOCs were sampled with a polydimethylsilicone (PDMS)-coupon and the resulting VOCs were recovered and analysed with two-stage thermal desorption gas chromatography-mass spectrometry. Fifteen young adult volunteers (19 years–26 years) participated in two interventions run in a randomised crossover design. One intervention, termed ‘Neutral’, required the participants to listen to peaceful music, the other, termed a ‘paced audio serial addition task’, required the participants to undertake a series of rapid mental arithmetic calculations in a challenging environment that induced a stress response. Skin-VOC samples were taken during each intervention. The resultant data were processed with dynamic background compensation, deconvolved, and registered to a common retention index scale. The importance of freezing skin patch samplers to −80 °C was determined during the method development phase of this study. The cumulative distribution function of the GC-MS data indicates the possibility that PDMS-coupons are selective towards the lower volatility VOC components in skin. The frequency distribution of the GC-MS data was observed to be approximately log-normal, and on the basis of this study, a further two-orders of magnitude reduction in sensitivity may be required before the complete skin-VOC profile may be characterised. Multi-variate analysis involving Pareto-scaling prior to partial least squares discriminant analysis identified four VOCs with the highest probability of contributing to the variance between the two states, and the responses to these VOCs were modelled with principle components analysis (PCA). Two VOCs, benzoic acid and n-decanoic acid were upregulated (14 and 8 fold respectively) and appear to be PASAT sensitive, with areas under (AUC) their receiver operator characteristic (ROC) curves of 0.813 and 0.852 respectively. A xylene isomer and 3-carene were down regulated 75% and 97% respectively, and found to be predictive of the neutral intervention (ROC AUC values of 0.898 and 0.929 respectively). VOC profiles in skin appear to change with stress either due to increased elimination, elevated bacterial activity, or perhaps increased oxidative pathways.
Description: This is an author-created, un-copyedited version of an article published in Journal of Breath Research. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/1752-7155/10/4/046012.
Sponsor: Unilever Research, Port Sunlight for the support of Helen Martin.
Version: Accepted for publication
DOI: 10.1088/1752-7155/10/4/046012
URI: https://dspace.lboro.ac.uk/2134/23669
Publisher Link: http://dx.doi.org/10.1088/1752-7155/10/4/046012
ISSN: 1752-7155
Appears in Collections:Published Articles (Sport, Exercise and Health Sciences)
Published Articles (Chemistry)

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