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Title: Liquid crystalline ordered collagen substrates for applications in tissue engineering
Authors: Price, Joshua C.
Roach, Paul
El Haj, Alicia J.
Keywords: Collagen
Liquid crystal
Self-assembly
Anisotropic
Tissue engineering
hMSC
Issue Date: 2016
Publisher: © American Chemical Society
Citation: PRICE, J.C., ROACH, P. and EL HAJ, A.J., 2016. Liquid crystalline ordered collagen substrates for applications in tissue engineering. ACS Biomaterials Science & Engineering, 2(4), pp. 625-633.
Abstract: This report describes methods for fabricating substrates with anisotropic order from a single solution of high concentration collagen. By exploiting the intrinsic property of collagen to behave as a cholesteric liquid crystal, we demonstrate first the production of dense collagen films containing anisotropic fibers by simple dialysis and polymerization in ammonia vapor. We then utilized shear driven alignment of collagen using viscous extrusion to produce aligned collagen fibers. Next we describe an evaporation technique to observe crystalline growth into the collagen, which serves to template the substrate prior to fibrillogenesis. The ordered substrates supported osteogenic differentiation of hMSCs and also oriented growth of hMSCs. We also demonstrate using Raman spectroscopy that the local protein concentration in the substrates influenced the molecular orientation of collagen, Finally, we compare the resultant textures in the substrates with section of native cornea and tendon using polarized light microscopy, which showed remarkable similarities in terms of both anisotropy and second order chiral structure. These rapid, cost-effective methods could potentially serve a range of different applications in tissue engineering.
Description: This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Biomaterials Science & Engineering, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsbiomaterials.6b00030
Sponsor: This research was funded by EPSRC DTC in Regenerative medicine, Keele University. Grant funding number: EP/F500491/1
Version: Accepted for Publication
DOI: 10.1021/acsbiomaterials.6b00030
URI: https://dspace.lboro.ac.uk/2134/23853
Publisher Link: http://dx.doi.org/10.1021/acsbiomaterials.6b00030
ISSN: 2373-9878
Appears in Collections:Published Articles (Physics)

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