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Title: Urinary extracellular vesicles: Potential biomarkers of renal function in diabetic patients
Authors: Kaminska, Agnieszka
Platt, Mark
Kasprzyk, Joanna
Kusnierz-Cabala, Beata
Gala-Bladzinska, Agnieszka
Woznicka, Olga
Jany, Benedykt R.
Krok, Franciszek
Piekoszewski, Wojciech
Kuzniewski, Marek
Stepien, Ewa L.
Issue Date: 2017
Publisher: © The Authors. Published by the Hindawi Publishing Corporation
Citation: KAMINSKA, A. ...et al., 2017. Urinary extracellular vesicles: Potential biomarkers of renal function in diabetic patients. Journal of Diabetes Research, 2016: 5741518.
Abstract: The aim of this study was to check the relationship between the density of urinary EVs, their size distribution, and the progress of early renal damage in type 2 diabetic patients (DMt2). Patients were enrolled to this study, and glycated hemoglobin (HbA1c) below 7% was a threshold for properly controlled diabetic patients (CD) and poorly controlled diabetic patients (UD). Patients were further divided into two groups: diabetic patients without renal failure (NRF) and with renal failure (RF) according to the Glomerular Filtration Rate. Density and diameter of EVs were determined by Tunable Resistive Pulse Sensing. Additionally, EVs were visualized by means of Transmission and Environmental Scanning Electron Microscopy. Nano-liquid chromatography coupled offline with mass spectrometry (MALDI-TOF-MS/MS) was applied for proteomic analysis. RF had reduced density of EVs compared to NRF.The size distribution study showed that CD had larger EVs (mode) than UD (115 versus 109 nm; 𝑝 < 0.05); nevertheless themean EVs diameter was smaller in controls than in the CD group (123 versus 134 nm; 𝑝 < 0.05). It was demonstrated that EVs are abundant in urine. Albumin, uromodulin, and number of unique proteins related to cell stress and secretion were detected in the EVs fraction. Density and size of urinary EVs reflect deteriorated renal function and can be considered as potential renal damage biomarkers.
Description: This is an Open Access Article. It is published by Hindawi Publishing Corporation under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/
Version: Published
DOI: 10.1155/2016/5741518
URI: https://dspace.lboro.ac.uk/2134/23874
Publisher Link: http://dx.doi.org/10.1155/2016/5741518
ISSN: 2314-6753
Appears in Collections:Published Articles (Chemistry)

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