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Title: Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation
Authors: Kasper, Andreas M.
Turner, Daniel C.
Martin, Neil R.W.
Sharples, Adam
Keywords: Skeletal muscle bioengineering
Satellite cells
Myoblasts
Electrical stimulation
Mechanical loading
Hypertrophy
Issue Date: 2017
Publisher: © Wiley
Citation: KASPER, A.M. ... et al, 2017. Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation. Journal of Cellular Physiology, doi:10.1002/jcp.25840.
Abstract: Bioengineering of skeletal muscle in-vitro in order to produce highly aligned myofibres in relevant three dimensional (3D) matrices have allowed scientists to model the in-vivo skeletal muscle niche. This review discusses essential experimental considerations for developing bioengineered muscle in order to investigate exercise mimicking stimuli. We identify current knowledge in the use of electrical stimulation and co-culture with motor neurons to enhance skeletal muscle maturation and contractile function in bioengineered systems in-vitro. Importantly, we provide a current opinion on the use of acute and chronic exercise mimicking stimuli (electrical stimulation and mechanical overload) and the subsequent mechanisms underlying physiological adaptation in 3D bioengineered muscle. We also identify that future studies using the latest bioreactor technology, providing simultaneous electrical and mechanical loading and flow perfusion in-vitro, may provide the basis for advancing knowledge in the future. We also envisage, that more studies using genetic, pharmacological and hormonal modifications applied in human 3D bioengineered skeletal muscle may allow for an enhanced discovery of the in-depth mechanisms underlying the response to exercise in relevant human testing systems. Finally, 3D bioengineered skeletal muscle may provide an opportunity to be used as a pre-clinical in-vitro test-bed to investigate the mechanisms underlying catabolic disease, whilst modelling disease itself via the use of cells derived from human patients without exposing animals or humans (in phase I trials) to the side effects of potential therapies.
Description: This paper is embargoed until twelve months after publication.
Sponsor: Authors would like to thank the Rugby Football Union, UK and the Society for Endocrinology for funding and supporting this work.
Version: Accepted for publication
DOI: 10.1002/jcp.25840
URI: https://dspace.lboro.ac.uk/2134/24235
Publisher Link: http://dx.doi.org/10.1002/jcp.25840
ISSN: 0021-9541
Appears in Collections:Closed Access (Sport, Exercise and Health Sciences)

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