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|Title: ||Increasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry|
|Authors: ||Arthur, Kayleigh L.|
Turner, Matthew A.
Reynolds, James C.
Creaser, Colin S.
|Keywords: ||Field asymmetric waveform ion mobility spectrometry|
Differential ion mobility spectrometry
Collision induced dissociation
|Issue Date: ||2017|
|Publisher: ||© American Chemical Society|
|Citation: ||ARTHUR, K.L. ... et al, 2017. Increasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry. Analytical Chemistry, 89 (6), pp. 3452-3459.|
|Abstract: ||Full scan field asymmetric waveform ion mobility spectrometry (FAIMS) combined with liquid chromatography and mass spectrometry (LC-FAIMS-MS) is shown to enhance peak capacity for omics applications. A miniaturized FAIMS device capable of rapid compensation field scanning has been incorporated into an ultrahigh performance liquid chromatography (UHPLC)
and time-of-flight mass spectrometry analysis, allowing the acquisition of full scan FAIMS and MS nested data sets within the time scale of a UHPLC peak. Proof of principle for the potential of scanning LC-FAIMS-MS in omics applications is demonstrated for the nontargeted profiling of human urine using a HILIC column. The high level of orthogonality between FAIMS and MS provides additional unique compound identifiers with detection of features based on retention time, FAIMS dispersion field and compensation field (DF and CF), and mass-to-charge (m/z). Extracted FAIMS full scan data can be matched to standards to aid the identification of unknown analytes. The peak capacity for features detected in human urine using LC-FAIMS-MS was increased approximately threefold compared to LC-MS alone due to a combination of the reduction of chemical noise and separation of coeluting isobaric species across the entire analytical space. The use of FAIMS-selected in source collision induced dissociation (FISCID) yields fragmentation of ions, which reduces sample complexity associated with overlapping fragmentation patterns and provides structural information on the selected precursor ions.|
|Description: ||This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.analchem.6b04315.|
|Sponsor: ||The authors thank Owlstone Limited and Loughborough University for financial support.|
|Version: ||Accepted for publication|
|Publisher Link: ||http://dx.doi.org/10.1021/acs.analchem.6b04315|
|Appears in Collections:||Published Articles (Chemistry)|
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