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Title: Increasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry
Authors: Arthur, Kayleigh L.
Turner, Matthew A.
Reynolds, James C.
Creaser, Colin S.
Keywords: Field asymmetric waveform ion mobility spectrometry
Differential ion mobility spectrometry
Liquid chromatography
HILIC
Mass spectrometry
Omics
Metabolomics
Proteomics
Non-targeted
Collision induced dissociation
Issue Date: 2017
Publisher: © American Chemical Society
Citation: ARTHUR, K.L. ... et al, 2017. Increasing peak capacity in nontargeted omics applications by combining full scan field asymmetric waveform ion mobility spectrometry with liquid chromatography–mass spectrometry. Analytical Chemistry, 89 (6), pp. 3452-3459.
Abstract: Full scan field asymmetric waveform ion mobility spectrometry (FAIMS) combined with liquid chromatography and mass spectrometry (LC-FAIMS-MS) is shown to enhance peak capacity for omics applications. A miniaturized FAIMS device capable of rapid compensation field scanning has been incorporated into an ultrahigh performance liquid chromatography (UHPLC) and time-of-flight mass spectrometry analysis, allowing the acquisition of full scan FAIMS and MS nested data sets within the time scale of a UHPLC peak. Proof of principle for the potential of scanning LC-FAIMS-MS in omics applications is demonstrated for the nontargeted profiling of human urine using a HILIC column. The high level of orthogonality between FAIMS and MS provides additional unique compound identifiers with detection of features based on retention time, FAIMS dispersion field and compensation field (DF and CF), and mass-to-charge (m/z). Extracted FAIMS full scan data can be matched to standards to aid the identification of unknown analytes. The peak capacity for features detected in human urine using LC-FAIMS-MS was increased approximately threefold compared to LC-MS alone due to a combination of the reduction of chemical noise and separation of coeluting isobaric species across the entire analytical space. The use of FAIMS-selected in source collision induced dissociation (FISCID) yields fragmentation of ions, which reduces sample complexity associated with overlapping fragmentation patterns and provides structural information on the selected precursor ions.
Description: This paper is closed access until 23rd February 2018. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.analchem.6b04315.
Sponsor: The authors thank Owlstone Limited and Loughborough University for financial support.
Version: Accepted for publication
DOI: 10.1021/acs.analchem.6b04315
URI: https://dspace.lboro.ac.uk/2134/24804
Publisher Link: http://dx.doi.org/10.1021/acs.analchem.6b04315
ISSN: 0003-2700
Appears in Collections:Closed Access (Chemistry)

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