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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/24935

Title: Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair
Authors: Donoghue, Peter S.
Sun, Tao
Gadegaard, Nikolaj
Riehle, Mathis O.
Barnett, Susan C.
Keywords: 3D culture
Neurons
Astrocytes
Fibroblasts
Mini-chamber
Microtopography
Issue Date: 2014
Publisher: © American Chemical Society
Citation: DONOGHUE, P.S. ... et al, 2014. Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair. Molecular Pharmaceutics, 11 (7), pp.2143-2150
Abstract: Tubular scaffolds which incorporate a variety of micro- and nanotopographies have a wide application potential in tissue engineering especially for the repair of spinal cord injury (SCI). We aim to produce metabolically active differentiated tissues within such tubes, as it is crucially important to evaluate the biological performance of the three-dimensional (3D) scaffold and optimize the bioprocesses for tissue culture. Because of the complex 3D configuration and the presence of various topographies, it is rarely possible to observe and analyze cells within such scaffolds in situ. Thus, we aim to develop scaled down mini-chambers as simplified in vitro simulation systems, to bridge the gap between two-dimensional (2D) cell cultures on structured substrates and three-dimensional (3D) tissue culture. The mini-chambers were manipulated to systematically simulate and evaluate the influences of gravity, topography, fluid flow, and scaffold dimension on three exemplary cell models that play a role in CNS repair (i.e., cortical astrocytes, fibroblasts, and myelinating cultures) within a tubular scaffold created by rolling up a microstructured membrane. Since we use CNS myelinating cultures, we can confirm that the scaffold does not affect neural cell differentiation. It was found that heterogeneous cell distribution within the tubular constructs was caused by a combination of gravity, fluid flow, topography, and scaffold configuration, while cell survival was influenced by scaffold length, porosity, and thickness. This research demonstrates that the mini-chambers represent a viable, novel, scale down approach for the evaluation of complex 3D scaffolds as well as providing a microbioprocessing strategy for tissue engineering and the potential repair of SCI.
Description: This is an ACS AuthorChoice article.
Sponsor: We gratefully acknowledge the financial support from BBSRC (U.K.) (grant number: BBG0047061) for this study.
Version: Published
DOI: 10.1021/mp400526n
URI: https://dspace.lboro.ac.uk/2134/24935
Publisher Link: http://dx.doi.org/10.1021/mp400526n
ISSN: 1543-8384
Appears in Collections:Published Articles (Chemical Engineering)

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