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|Title: ||The Development of a ɛ-Polycaprolactone Scaffold for Central Nervous System Repair|
|Authors: ||Donoghue, Peter S.|
Boomkamp, Stephanie D.
Riehle, Mathis O.
Barnett, Susan C.
|Issue Date: ||2013|
|Publisher: ||© Mary Ann Liebert, Inc. publishers|
|Citation: ||DONOGHUE, P.S. ... et al, 2013. The Development of a ɛ-Polycaprolactone Scaffold for Central Nervous System Repair. Tissue Engineering Part A, 19 (3-4), pp.497-507|
|Abstract: ||Potential treatment strategies for the repair of spinal cord injury (SCI)
currently favor a combinatorial approach incorporating several factors,
including exogenous cell transplantation and biocompatible scaffolds.
The use of scaffolds for bridging the gap at the injury site is very
appealing although there has been little investigation into the central
nervous system neural cell interaction and survival on such scaffolds
before implantation. Previously, we demonstrated that aligned
microgrooves 12.5-25 μm wide on ε-polycaprolactone (PCL) promoted
aligned neurite orientation and supported myelination. In this study, we
identify the appropriate substrate and its topographical features required
for the design of a three-dimensional scaffold intended for
transplantation in SCI. Using an established myelinating culture system
of dissociated spinal cord cells, recapitulating many of the features of
the intact spinal cord, we demonstrate that astrocytes plated on the
topography secrete soluble factors(s) that delay oligodendrocyte
differentiation, but do not prevent myelination. However, as myelination
does occur after a further 10-12 days in culture, this does not prevent
the use of PCL as a scaffold material as part of a combined strategy for
the repair of SCI.|
|Description: ||This paper is closed access.|
|Sponsor: ||The work described in the article was funded by the
Biotechnology and Biological Sciences Research Council
PCL SCAFFOLD FOR CNS REPAIR 505
(BB/G004706/1) (BBSRC, PD, ST); Lord Kelvin and Adam
Smith PhD studentship (RL) and NC3Rs (SDB).|
|Version: ||Closed access|
|Publisher Link: ||http://dx.doi.org/10.1089/ten.tea.2012.0382|
|Appears in Collections:||Closed Access (Chemical Engineering)|
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