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Development of a mini 3D cell culture system using well defined nickel grids for the investigation of cell scaffold interactions
journal contribution
posted on 2017-05-12, 11:21 authored by Tao SunTao Sun, Rod Smallwood, Sheila MacNeilA bioreactor system was developed using a series of fine mesh nickel grids as free standing scaffolds to investigate the behaviours of fibroblasts and keratinocytes in tissue culture. It was found that the mesh size of the suspended grids, but not of the grids that attached to tissue culture surface, had significant influences on cell behaviour and there was a maximum size for fibroblast to span within the defined culture period. Time lapse video microscopy demonstrated fibroblasts cultured on these grids initially migrated onto the struts but then worked together to fill in the voids between struts with a membranous sheet of tissue. In contrast keratinocytes barely migrated from the initial site of cell deposition and when they moved (to a modest extent) it was as an integrated sheet of cells. Similar results were observed when both types of cells were co-cultured in the system.
Funding
This work was financially supported by the EPSRC.
History
School
- Aeronautical, Automotive, Chemical and Materials Engineering
Department
- Chemical Engineering
Published in
Journal of Materials Science: Materials in MedicineVolume
20Issue
7Pages
1483 - 1493Citation
SUN, T., SMALLWOOD, R. and MACNEIL, S., 2009. Development of a mini 3D cell culture system using well defined nickel grids for the investigation of cell scaffold interactions. Journal of Materials Science: Materials in Medicine, 20 (7), pp.1483-1493Publisher
© Springer Science+Business Media, LLCVersion
- NA (Not Applicable or Unknown)
Publisher statement
This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/Publication date
2009Notes
This paper is closed access.ISSN
0957-4530eISSN
1573-4838Publisher version
Language
- en