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Development of a closed bioreactor system for culture of tissue-engineered skin at an air–liquid interface

journal contribution
posted on 2017-05-12, 13:31 authored by Tao SunTao Sun, David Norton, John W. Haycock, Anthony J. Ryan, Sheila MacNeil
A bioreactor has been developed for the production of tissue-engineered skin at an air–liquid interface for clinical and experimental use. In this closed system, scaffold and bioreactor sterilization, cell seeding, and medium perfusion were all performed with a peristaltic pump. Natural and synthetic dermal substitutes were seeded directly with skin cells without opening the bioreactor and fed either by continuous medium perfusion or by batch-feed. The system was validated by monoculture of human dermal fibroblasts and keratinocytes and the coculture of both cell types in acellular human dermis, Azowipes, electrospun polystyrene, and an electrospun composite of polystyrene and poly-DL-lactide fibers. A comparison was made of culture at an air–liquid interface versus submerged culture and of medium change by continuous perfusion versus batch-feed. Fibroblast and endothelial cells showed greater viability under submerged rather than air–liquid conditions whereas keratinocytes favored culture at an air–liquid interface as did cocultured keratinocytes and fibroblasts. Total cellular viability for reconstructed skin with keratinocytes and fibroblasts was greatest with continuous perfusion rather than batch-feed and with electrospun scaffolds compared with acellular human dermis. The bioreactor could also be easily configured to give replicate small areas for experimental use or one continuous area of construct for clinical use.

History

School

  • Aeronautical, Automotive, Chemical and Materials Engineering

Department

  • Chemical Engineering

Published in

Tissue Engineering

Volume

11

Issue

11-12

Pages

1824 - 1831

Citation

SUN, T., 2005. Development of a closed bioreactor system for culture of tissue-engineered skin at an air–liquid interface. Tissue Engineering, 11 (11-12), pp.1824-1831

Publisher

© Mary Ann Liebert, Inc.

Version

  • NA (Not Applicable or Unknown)

Publisher statement

This work is made available according to the conditions of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) licence. Full details of this licence are available at: https://creativecommons.org/licenses/by-nc-nd/4.0/

Publication date

2005

Notes

This paper is closed access.

ISSN

1076-3279

eISSN

1557-8690

Language

  • en

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