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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/24969

Title: Development of a closed bioreactor system for culture of tissue-engineered skin at an air–liquid interface
Authors: Sun, Tao
Norton, David
Haycock, John W.
Ryan, Anthony J.
MacNeil, Sheila
Issue Date: 2005
Publisher: © Mary Ann Liebert, Inc.
Citation: SUN, T., 2005. Development of a closed bioreactor system for culture of tissue-engineered skin at an air–liquid interface. Tissue Engineering, 11 (11-12), pp.1824-1831
Abstract: A bioreactor has been developed for the production of tissue-engineered skin at an air–liquid interface for clinical and experimental use. In this closed system, scaffold and bioreactor sterilization, cell seeding, and medium perfusion were all performed with a peristaltic pump. Natural and synthetic dermal substitutes were seeded directly with skin cells without opening the bioreactor and fed either by continuous medium perfusion or by batch-feed. The system was validated by monoculture of human dermal fibroblasts and keratinocytes and the coculture of both cell types in acellular human dermis, Azowipes, electrospun polystyrene, and an electrospun composite of polystyrene and poly-DL-lactide fibers. A comparison was made of culture at an air–liquid interface versus submerged culture and of medium change by continuous perfusion versus batch-feed. Fibroblast and endothelial cells showed greater viability under submerged rather than air–liquid conditions whereas keratinocytes favored culture at an air–liquid interface as did cocultured keratinocytes and fibroblasts. Total cellular viability for reconstructed skin with keratinocytes and fibroblasts was greatest with continuous perfusion rather than batch-feed and with electrospun scaffolds compared with acellular human dermis. The bioreactor could also be easily configured to give replicate small areas for experimental use or one continuous area of construct for clinical use.
Description: This paper is closed access.
Version: Closed Access
DOI: 10.1089/ten.2005.11.1824
URI: https://dspace.lboro.ac.uk/2134/24969
Publisher Link: http://dx.doi.org/10.1089/ten.2005.11.1824
ISSN: 1076-3279
Appears in Collections:Closed Access (Chemical Engineering)

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