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Title: Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography
Authors: Sun, Tao
Chen, Ge
Liu, Yiping
Bu, Fengrong
Wen, Meijuan
Issue Date: 2000
Citation: SUN, T. ... et al, 2000. Purification of human prothrombin from Nitschmann fraction III using DEAE membrane radial flow chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 742 (1), pp.109-114
Abstract: A DEAE membrane radial column (4x5 cm) was used to purify human prothrombin from Nitschmann fraction III and performances of the column were investigated. Sample flow-rate of 20-30 ml/min (i.e., 24-36 bed volumes/h) and elution velocity up to 40 ml/min (i.e., 48 bed volumes/h) were obtained without compromising the separation efficiency. Its breakthrough capacity was one-third to half that of the commercial DEAE Sepharose Fast Flow media. It was concluded that the novel column is an attractive alternate to traditional axial column.
Description: This paper is Closed Access.
Version: Closed access
DOI: 10.1016/S0378-4347(00)00147-X
URI: https://dspace.lboro.ac.uk/2134/24978
Publisher Link: http://dx.doi.org/10.1016/S0378-4347(00)00147-X
ISSN: 0378-4347
Appears in Collections:Closed Access (Chemical Engineering)

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