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Title: Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph
Authors: Sun, Tao
Liu, Yiping
Bu, Fengrong
Chen, Ge
Wen, Meijuan
Keywords: Membrane radial column chromatography
Nitschmann fraction Ⅲ
Prothrombin complex concentrate
Purification
Issue Date: 2000
Publisher: Kexue Chubanshe (© Chinese Academic Journal Electronic Publishing House)
Citation: SUN, T. ... et al, 2000. Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph. Sepu = Chinese Journal of Chromatography / Zhongguo hua xue hui, 18, pp.350-353
Abstract: method is described for the purification of human prothrombin complex concentrate ( PCC) from Nitschmann fraction Ⅲ by membrane radial column ion2exchange chromatography , which allows large sample volumes to be processed at low operation pressure. The Nitschmann fraction Ⅲ(15 g) was mixed with 1 000 mL 0. 06 mol/ L Tris2HCl (pH 7. 5) . The centrifuged supernatant (10 000 r/ min ,15 min ,20 ℃) was applied onto a DEAE ion2exchange liquid chromatographic column (XK216 DEAE fast flow Sepharose and DEAE membrane radical column chromatography) with almost the same excellent separation efficiency. The parameters of sample flow rate , elution flow rate and sample capacity were optimized.
Description: This paper is closed access.
Version: Closed access
URI: https://dspace.lboro.ac.uk/2134/24983
Publisher Link: http://www.chrom-china.com/
http://www.cnki.net
ISSN: 1872-2059
Appears in Collections:Closed Access (Chemical Engineering)

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