Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Loughborough University Institutional Repository

Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/24983

Title: Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph
Authors: Sun, Tao
Liu, Yiping
Bu, Fengrong
Chen, Ge
Wen, Meijuan
Keywords: Membrane radial column chromatography
Nitschmann fraction Ⅲ
Prothrombin complex concentrate
Issue Date: 2000
Publisher: Kexue Chubanshe (© Chinese Academic Journal Electronic Publishing House)
Citation: SUN, T. ... et al, 2000. Purification of prothrombin in Nitschmann fraction III by membrane radial column ion-exchange liquid chromatograph. Sepu = Chinese Journal of Chromatography / Zhongguo hua xue hui, 18, pp.350-353
Abstract: method is described for the purification of human prothrombin complex concentrate ( PCC) from Nitschmann fraction Ⅲ by membrane radial column ion2exchange chromatography , which allows large sample volumes to be processed at low operation pressure. The Nitschmann fraction Ⅲ(15 g) was mixed with 1 000 mL 0. 06 mol/ L Tris2HCl (pH 7. 5) . The centrifuged supernatant (10 000 r/ min ,15 min ,20 ℃) was applied onto a DEAE ion2exchange liquid chromatographic column (XK216 DEAE fast flow Sepharose and DEAE membrane radical column chromatography) with almost the same excellent separation efficiency. The parameters of sample flow rate , elution flow rate and sample capacity were optimized.
Description: This paper is closed access.
Version: Closed access
URI: https://dspace.lboro.ac.uk/2134/24983
Publisher Link: http://www.chrom-china.com/
ISSN: 1872-2059
Appears in Collections:Closed Access (Chemical Engineering)

Files associated with this item:

File Description SizeFormat
膜径向离子交换色谱分离凝血酶原复合物.pdfPublished version184.21 kBAdobe PDFView/Open


SFX Query

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.