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Title: Chromatography of human prothrombin from Nitschmann fraction III on Q Sepharose Fast Flow using axial and radial flow column
Authors: Sun, Tao
Chen, Ge
Liu, Yipin
Bu, Fengrong
Wen, Meijuan
Issue Date: 2000
Publisher: © Springer-Verlag Berlin Heidelberg
Citation: SUN, T. ... et al, 2000. Chromatography of human prothrombin from Nitschmann fraction III on Q Sepharose Fast Flow using axial and radial flow column. Bioprocess Engineering, 23 (2), pp.121-125
Abstract: An axial column (Hitrap Q 5 ml, 2.5 × 1.6 cm) and a radial flow column (3.5 × 5 cm) packed with Q Sepharose Fast Flow media had been evaluated for the separation of human prothrombin. Nitschmann fraction III dissolved in buffered saline (0.10 M sodium chloride buffered with 0.06 M Tris/HCl to pH 7.5) was the starting material. Effects of sample flow rate of the two columns were screened. Under radial flow conditions using the radial column, sample flow rate up to 15 ml/min (i.e. 18 bed volumes/h) was achieved and the operating pressure was below 0.2 MPa eventhough the elution velocity was 30 ml/min. Breakthrough capacity was determined by analyzing the total protein and prothrombin activity of the target protein-containing fraction under subsaturating conditions and both columns had almost the same breakthrough capacity per ml media, indicating that the sample loading was independent of radial column geometry. It was concluded that the radial column is an attractive alternate to traditional axial packed bed column, exhibiting very good potential for use in the separation of human prothrombin.
Description: This paper is closed access.
Version: Closed access
DOI: 10.1007/s004499900132
URI: https://dspace.lboro.ac.uk/2134/24985
Publisher Link: http://dx.doi.org/10.1007/s004499900132
ISSN: 1615-7591
Appears in Collections:Closed Access (Chemical Engineering)

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