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Title: Isolation of human fibrinogen by axial and radial flow chromatography from Nitschmann fraction I
Authors: Sun, Tao
Chen, Ge
Liu, Yipin
Bu, Fengrong
Wen, Meijuan
Keywords: Axial chromatography
Human fibrinogen
Nitschmann fraction I
Radial flow chromatography
Issue Date: 1999
Publisher: Springer Verlag (© Kluwer Academic Publishers)
Citation: SUN, T. ... et al, 1999. Isolation of human fibrinogen by axial and radial flow chromatography from Nitschmann fraction I. Biotechnology Techniques, 13 (12), pp.831-835
Abstract: An axial column (3×2.6 cm) and a radial flow column (3.5×5 cm) packed with DEAE Sepharose Fast Flow media had been evaluated for the separation of human fibrinogen. Nitschmann fraction I dissolved in buffered saline (0.015 M NaCl buffered with 0.06 M Tris/HCl to pH 7.5) was the starting material. Under radial flow conditions, sample flow up to 15 ml min−1 (i.e., 18 bed volumes h−1) was achieved. The operating pressures were below 0.2 MPa, even though the elution velocity was 30 ml min−1 (i.e., 36 bed volumes h−1).
Description: This paper is closed access.
Version: Closed access
DOI: 10.1023/A:1008935623946
URI: https://dspace.lboro.ac.uk/2134/24987
Publisher Link: http://dx.doi.org/10.1023/A:1008935623946
ISSN: 0951-208X
Appears in Collections:Closed Access (Chemical Engineering)

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