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Title: Development of an optical system for the non-invasive tracking of stem cell growth on microcarriers
Authors: Odeleye, Akinlolu O.O.
Castillo-Avila, Sara
Boon, Mathew
Martin, Haydn
Coopman, Karen
Issue Date: 2017
Publisher: Wiley
Citation: ODELEYE, A.O.O. ... et al., 2017. Development of an optical system for the non-invasive tracking of stem cell growth on microcarriers. Biotechnology and Bioengineering, First published: 23 May 2017, DOI: 10.1002/bit.26328
Abstract: The emergence of medicinal indications for stem cell therapies has seen a need to develop the manufacturing capacity for adherent cells such as mesenchymal stem cells (MSCs). One such development is in the use of microcarriers, which facilitate enhanced cell densities for adherent stem cell cultures when compared with 2D culture platforms. Given the variety of stem cell expansion systems commercially available, novel methods of non-invasive and automated monitoring of cell number, confluence, and aggregation, within disparate environments, will become imperative to process control, ensuring reliable and consistent performance. The in situ epiillumination of mouse embryonic fibroblasts and human mesenchymal stem cells attached to Cytodex 1 and 3 microcarriers was achieved using a bespoke microscope. Robust image processing techniques were developed to provide quantitative measurements of confluence, aggregate recognition, and cell number, without the need for fluorescent labeling or cell detachment. Large datasets of cells counted on individual microcarriers were statistically analyzed and compared with NucleoCounter measurements, with an average difference of less than 7% observed from days 0 to 6 of a 12-day culture noted, prior to the onset of aggregation. The developed image acquisition system and postprocessing methodologies were successfully applied to dynamically moving colonized microcarriers. The proposed system offers a novel method of cell identification at the individual level, to consistently and accurately assess viable cell number, confluence, and cell distribution, while also minimizing the variability inherent in the current invasive means by which cells adhered to microcarriers are analyzed.
Description: This work is made available according to the conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0) licence. Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/
Sponsor: Engineering and Physical Sciences Research Council [grant numbers: EP/L017555/1 EP/L017571/1]
Version: Published
DOI: 10.1002/bit.26328
URI: https://dspace.lboro.ac.uk/2134/25121
Publisher Link: http://dx.doi.org/10.1002/bit.26328
ISSN: 0006-3592
Appears in Collections:Published Articles (Chemical Engineering)

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