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Title: Physical activity, immune function and inflammation in kidney patients (the PINK study): a feasibility trial protocol
Authors: Highton, Patrick James
Neale, Jill
Wilkinson, Thomas J.
Bishop, Nicolette
Smith, Alice C.
Issue Date: 2017
Publisher: © The Authors. Published by BMJ Publishing Group
Citation: HIGHTON, P.J. ...et al., 2017. Physical activity, immune function and inflammation in kidney patients (the PINK study): a feasibility trial protocol. BMJ Open, 7(5): e014713.
Abstract: Introduction Patients with chronic kidney disease (CKD) display increased infection-related mortality and elevated cardiovascular risk only partly attributed to traditional risk factors. Patients with CKD also exhibit a pro-inflammatory environment and impaired immune function. Aerobic exercise has the potential to positively impact these detriments, but is under-researched in this patient population. This feasibility study will investigate the effects of acute aerobic exercise on inflammation and immune function in patients with CKD to inform the design of larger studies intended to ultimately influence current exercise recommendations. Methods and analysis Patients with CKD, including renal transplant recipients, will visit the laboratory on two occasions, both preceded by appropriate exercise, alcohol and caffeine restrictions. On visit 1, baseline assessments will be completed, comprising anthropometrics, body composition, cardiovascular function and fatigue and leisure time exercise questionnaires. Participants will then undertake an incremental shuttle walk test to estimate predicted peak O2 consumption (VO2 peak). On visit 2, participants will complete a 20 min shuttle walk at a constant speed to achieve 85% estimated VO2 peak. Blood and saliva samples will be taken before, immediately after and 1 hour after this exercise bout. Muscle O2 saturation will be monitored throughout exercise and recovery. Age and sex-matched non-CKD ‘healthy control’ participants will complete an identical protocol. Blood and saliva samples will be analysed for markers of inflammation and immune function, using cytometric bead array and flow cytometry techniques. Appropriate statistical tests will be used to analyse the data. Ethics and dissemination A favourable opinion was granted by the East Midlands-Derby Research Ethics Committee on 18 September 2015 (ref 15/EM/0391), and the study was approved and sponsored by University Hospitals of Leicester Research and Innovation (ref 11444). The study was registered with ISRCTN (ref 38935454). The results will be presented at relevant conferences, and it is anticipated that the reports will be published in appropriate journals in 2018.
Description: This is an Open Access Article. It is published by BMJ publishing group under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/
Sponsor: This research was partially funded by the Stoneygate Trust. This work is supported by the National Institute for Health Research (NIHR) Diet, Lifestyle & Physical Activity Biomedical Research Unit based at University Hospitals of Leicester and Loughborough University.
Version: Published
DOI: 10.1136/bmjopen-2016-014713
URI: https://dspace.lboro.ac.uk/2134/25363
Publisher Link: http://dx.doi.org/10.1136/bmjopen-2016-014713
ISSN: 2044-6055
Appears in Collections:Published Articles (Sport, Exercise and Health Sciences)

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