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Title: Automated adherent human cell culture (mesenchymal stem cells)
Authors: Thomas, Robert James
Ratcliffe, Elizabeth
Keywords: Alkaline phosphatase
Automation
Flow cytometry
Humans
Mesenchymal stem cells
Osteogenesis
Stem cells
Issue Date: 2012
Publisher: © Springer
Citation: THOMAS, R. and RATCLIFFE, E., 2012. Automated adherent human cell culture (mesenchymal stem cells). IN: Mitry, R. and Hughes, R. (eds.). Human Cell Culture Protocols. (Methods in Molecular Biology ; vol. 806.) London: Springer, pp.393-406.
Series/Report no.: Methods in Molecular Biology;806
Abstract: Human cell culture processes developed at research laboratory scale need to be translated to large-scale production processes to achieve commercial application to a large market. To allow this transition of scale with consistent process performance and control of costs, it will be necessary to reduce manual processing and increase automation. There are a number of commercially available platforms that will reduce manual process intervention and improve process control for different culture formats. However, in many human cell-based applications, there is currently a need to remain close to the development format, usually adherent culture on cell culture plastic or matrix-coated wells or flasks due to deterioration of cell quality in other environments, such as suspension. This chapter presents an example method for adherent automated human stem cell culture using a specific automated flask handling platform, the CompacT SelecT.
Version: Not specified
DOI: 10.1007/978-1-61779-367-7_26
URI: https://dspace.lboro.ac.uk/2134/26155
Publisher Link: http://dx.doi.org/10.1007/978-1-61779-367-7_26
ISBN: 9781617793660
9781617793677
ISSN: 1064-3745
Appears in Collections:Closed Access (Chemical Engineering)

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