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|Title: ||Development of a UHPLC-MS/MS (SRM) method for the quantitation of endogenous glucagon and dosed GLP-1 from human plasma|
|Authors: ||Howard, James W.|
Kay, Richard G.
Creaser, Colin S.
Supercharging mobile phase additive
|Issue Date: ||2017|
|Publisher: ||© 2017 Future Science Ltd|
|Citation: ||HOWARD, J.W. ...et al., 2017. Development of a UHPLC-MS/MS (SRM) method for the quantitation of endogenous glucagon and dosed GLP-1 from human plasma. Bioanalysis, 9(9), pp. 733-751.|
|Abstract: ||© 2017 Future Science Ltd. Aim: The performance of glucagon and GLP-1 immunoassays is often poor, but few sensitive LC-MS/MS methods exist as alternatives. Experimental: A multiplexed LC-MS/MS method using a 2D extraction technique was developed. Results: The method was established for the quantitation of endogenous glucagon (LLOQ: 15 pg/ml) and dosed GLP-1 (LLOQ: 25 pg/ml) in human plasma, and is the first such method avoiding immunoenrichment. Specificity of endogenous glucagon quantitation was assured using a novel approach with a supercharging mobile phase additive to access a sensitive qualifier SRM. Endogenous glucagon concentrations were within the expected range, and showed good reproducibility after extended sample storage. A cross-validation against established immunoassays using physiological study samples demonstrated some similarities between methods. Conclusion: The LC-MS/MS method offers a viable alternative to immunoassays for quantitation of endogenous glucagon, dosed glucagon and/or dosed GLP-1.|
|Description: ||This paper was published in the journal Bioanalysis and the definitive published version is available at http://dx.doi.org/10.4155/bio-2017-0021.|
|Version: ||Accepted for publication|
|Publisher Link: ||http://dx.doi.org/10.4155/bio-2017-0021|
|Appears in Collections:||Published Articles (Chemistry)|
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