HEWITT, S.H. ... et al, 2017. A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex. Chemical Communications, 53 (94), pp. 12626-12629.
We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
This paper was published in the journal Chemical Communications and the definitive published version is available at https://doi.org/10.1039/c7cc05887j.
This work was supported by a Wellcome Trust Seed Award (204500/Z/16/Z) and a Royal Society Research Grant.