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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/26695

Title: A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex
Authors: Hewitt, Sarah H.
Parris, Jonathan
Mailhot, Romain
Butler, Stephen J.
Issue Date: 2017
Publisher: © Royal Society of Chemistry
Citation: HEWITT, S.H. ... et al, 2017. A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex. Chemical Communications, In Press.
Abstract: We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
Description: This paper is closed access until 12 months after publication.
Sponsor: This work was supported by a Wellcome Trust Seed Award (204500/Z/16/Z) and a Royal Society Research Grant.
Version: Accepted for publication
DOI: 10.1039/c7cc05887j
URI: https://dspace.lboro.ac.uk/2134/26695
Publisher Link: https://doi.org/10.1039/c7cc05887j
ISSN: 1359-7345
Appears in Collections:Closed Access (Chemistry)

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