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Title: Laser ablation-inductively coupled plasma mass spectrometry: An emerging technology for detecting rare cells in tissue sections
Authors: Managh, Amy J.
Hutchinson, Robert W.
Riquelme, Paloma
Broichhausen, Christiane
Wege, Anja K.
Ritter, Uwe
Ahrens, Norbert
Koehl, Gudrun E.
Walter, Lisa
Florian, Christian
Schlitt, Hans J.
Reid, Helen J.
Geissler, Edward K.
Sharp, Barry L.
Hutchinson, James A.
Issue Date: 2014
Publisher: American Association of Immunologists
Citation: MANAGH, A.J., 2014. Laser ablation-inductively coupled plasma mass spectrometry: An emerging technology for detecting rare cells in tissue sections. Journal of Immunology, 193(5), pp. 2600 - 2608.
Abstract: © 2014 by The American Association of Immunologists, Inc. Administering immunoregulatory cells to patients as medicinal agents is a potentially revolutionary approach to the treatment of immunologically mediated diseases. Presently, there are no satisfactory, clinically applicable methods of tracking human cells in patients with adequate spatial resolution and target cell specificity over a sufficient period of time. Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS) represents a potential solution to the problem of detecting very rare cells in tissues. In this article, this exquisitely sensitive technique is applied to the tracking of gold-labeled human regulatory macrophages (Mregs) in immunodeficient mice. Optimal conditions for labeling Mregs with 50-nm gold particles were investigated by exposing Mregs in culture to variable concentrations of label: Mregs incubated with 3.5 × 10 9 particles/ml for 1 h incorporated an average of 3.39 × 10 8 Au atoms/cell without loss of cell viability. Analysis of single, gold-labeled Mregs by LA-ICP-MS registered an average of 1.9 × 10 5 counts/cell. Under these conditions, 100% labeling efficiency was achieved, and label was retained by Mregs for ≥36 h. Gold-labeled Mregs adhered to glass surfaces; after 24 h of culture, it was possible to colabel these cells with humanspecific 154 Sm-tagged anti-HLA-DR or 174 Yb-tagged anti-CD45 mAbs. Following injection into immunodeficient mice, signals from gold-labeled human Mregs could be detected in mouse lung, liver, and spleen for at least 7 d by solution-based inductively coupled plasma mass spectrometry and LA-ICP-MS. These promising results indicate that LA-ICP-MS tissue imaging has great potential as an analytical technique in immunology. Copyright
Description: This paper is in closed access.
Version: Accepted for publication
DOI: 10.4049/jimmunol.1400869
URI: https://dspace.lboro.ac.uk/2134/26717
Publisher Link: http://dx.doi.org/10.4049/jimmunol.1400869
ISSN: 0022-1767
Appears in Collections:Closed Access (Chemistry)

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