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Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/26784

Title: Development of a high-throughput UHPLC-MS/MS (SRM) method for the quantitation of endogenous glucagon from human plasma
Authors: Howard, James W.
Kay, Richard G.
Tan, Tricia
Minnion, James
Ghatei, Mohammad
Bloom, Steve
Creaser, Colin S.
Issue Date: 2014
Publisher: © Future Science Ltd
Citation: HOWARD, J.W. ...et al., 2014. Development of a high-throughput UHPLC-MS/MS (SRM) method for the quantitation of endogenous glucagon from human plasma. Bioanalysis, 6(24), pp. 3295-3309.
Abstract: © 2014 Future Science Ltd. Background: Published LC-MS/MS methods are not sensitive enough to quantify endogenous levels of glucagon. Results: An ultra high performance liquid chromatography-MS/MS (SRM) method for the quantitation of endogenous levels glucagon was successfully developed and qualified. A novel 2D extraction procedure was used to reduce matrix suppression, background noise and interferences. Glucagon levels in samples from healthy volunteers were found to agree with radioimmunoassay (RIA) derived literature values. Bland-Altman analysis showed a concentration-dependent positive bias of the LC/MS-MS assay versus an RIA. Both assays produced similar pharmacokinetic profiles, both of which were feasible considering the nature of the study. Conclusion: Our method is the first peer reviewed LC-MS/MS method for the quantitation of endogenous levels of glucagon, and offers a viable alternative to RIA-based approaches.
Description: This paper was accepted for publication in the journal Bioanalysis and the definitive published version is available at http://dx.doi.org/10.4155/bio.14.226
Version: Accepted for publication
DOI: 10.4155/bio.14.226
URI: https://dspace.lboro.ac.uk/2134/26784
Publisher Link: http://dx.doi.org/10.4155/bio.14.226
ISSN: 1757-6180
Appears in Collections:Published Articles (Chemistry)

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