Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Loughborough University Institutional Repository

Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/27211

Title: Annexin-enriched osteoblast-derived vesicles act as an extracellular site of mineral nucleation within developing stem cell cultures
Authors: Davies, O.G.
Cox, S.C.
Williams, R.L.
Tsaroucha, D.
Dorrepaal, R.M.
Lewis, Mark P.
Grover, L.M.
Issue Date: 2017
Publisher: © the Authors. Published by the Nature Publishing Group
Citation: DAVIES, O.G. ...et al., 2017. Annexin-enriched osteoblast-derived vesicles act as an extracellular site of mineral nucleation within developing stem cell cultures. Scientific Reports, 7: 12639.
Abstract: The application of extracellular vesicles (EVs) as natural delivery vehicles capable of enhancing tissue regeneration could represent an exciting new phase in medicine. We sought to define the capacity of EVs derived from mineralising osteoblasts (MO-EVs) to induce mineralisation in mesenchymal stem cell (MSC) cultures and delineate the underlying biochemical mechanisms involved. Strikingly, we show that the addition of MO-EVs to MSC cultures significantly (P < 0.05) enhanced the expression of alkaline phosphatase, as well as the rate and volume of mineralisation beyond the current gold-standard, BMP-2. Intriguingly, these effects were only observed in the presence of an exogenous phosphate source. EVs derived from non-mineralising osteoblasts (NMO-EVs) were not found to enhance mineralisation beyond the control. Comparative label-free LC-MS/MS profiling of EVs indicated that enhanced mineralisation could be attributed to the delivery of bridging collagens, primarily associated with osteoblast communication, and other non-collagenous proteins to the developing extracellular matrix. In particular, EV-associated annexin calcium channelling proteins, which form a nucleational core with the phospholipid-rich membrane and support the formation of a pre-apatitic mineral phase, which was identified using infrared spectroscopy. These findings support the role of EVs as early sites of mineral nucleation and demonstrate their value for promoting hard tissue regeneration.
Description: This is an Open Access Article. It is published by Nature under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/
Sponsor: The work was directly funded by an EPSRC E-TERM Landscape fellowship personally awarded to Dr. Owen Davies. R.M. Dorrepaal was funded by the European Research Council (ERC) under the starting grant programme ERC-2013-StG call—Proposal No. 335508—BioWater.
Version: Published
DOI: 10.1038/s41598-017-13027-6
URI: https://dspace.lboro.ac.uk/2134/27211
Publisher Link: https://doi.org/10.1038/s41598-017-13027-6
ISSN: 2045-2322
Appears in Collections:Published Articles (Sport, Exercise and Health Sciences)

Files associated with this item:

File Description SizeFormat
Davies_s41598-017-13027-6.pdfPublished version2.47 MBAdobe PDFView/Open

 

SFX Query

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.