Chemiluminescence and fluorescence immunoassays are increasingly
being employed in analytical sciences in order to circumvent
problems associated with radioimmunoassays. Fluorescence immunoassays
in practice suffer from background interferences, and have
inferior limits of detection. Chemical excitation of conventional
fluorescent labels via peroxyoxalate offers an attractive approach
for improving the detection limits of fluorescence immunoassays. [Continues.]
A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy at Loughborough University.