Loughborough University
Leicestershire, UK
LE11 3TU
+44 (0)1509 263171
Loughborough University

Loughborough University Institutional Repository

Please use this identifier to cite or link to this item: https://dspace.lboro.ac.uk/2134/28269

Title: Neural and aneural regions generated by the use of chemical surface coatings
Authors: Pardo-Figuerez, Maria M.
Martin, Neil R.W.
Player, Darren J.
Capel, Andrew J.
Christie, Steven D.R.
Lewis, Mark P.
Keywords: Cell behavior
Polymer brushes
SAMs
SH-SY5Y
Issue Date: 2017
Publisher: © American Chemical Society
Citation: PARDO-FIGUEREZ, M.M. ... et al, 2017. Neural and aneural regions generated by the use of chemical surface coatings. ACS Biomaterials Science and Engineering, 4 (1), pp. 98–106.
Abstract: The disordered environment found in conventional neural cultures impedes various applications where cell directionality is a key process for functionality. Neurons are highly specialized cells known to be greatly dependent on interactions with their surroundings. Therefore, when chemical cues are incorporated on the surface material, a precise control over neuronal behavior can be achieved. Here, the behavior of SH-SY5Y neurons on a variety of self-assembled monolayers (SAMs) and polymer brushes both in isolation and combination to promote cellular spatial control was determined. APTES and BIBB coatings promoted the highest cell viability, proliferation, metabolic activity, and neuronal maturation, while low cell survival was seen on PKSPMA and PMETAC surfaces. These cell-attractive and repulsive surfaces were combined to generate a binary BIBB-PKSPMA coating, whereby cellular growth was restricted to an exclusive neural region. The utility of these coatings when precisely combined could act as a bioactive/bioinert surface resulting in a biomimetic environment where control over neuronal growth and directionality can be achieved.
Description: This is an Open Access Article. It is published by the American Chemical Society under the Creative Commons Attribution 4.0 International Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/
Sponsor: We thank Loughborough University and EPSRC (Grant EP/L02067X/1) for financially supporting this project.
Version: Published
DOI: 10.1021/acsbiomaterials.7b00663
URI: https://dspace.lboro.ac.uk/2134/28269
Publisher Link: https://doi.org/10.1021/acsbiomaterials.7b00663
ISSN: 2373-9878
Appears in Collections:Published Articles (Chemistry)
Published Articles (Sport, Exercise and Health Sciences)

Files associated with this item:

File Description SizeFormat
acsbiomaterials.7b00663.pdfPublished version5.22 MBAdobe PDFView/Open

 

SFX Query

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.