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|Title: ||The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction.|
|Authors: ||Ferguson, Richard A.|
Hunt, Julie E.A.
Lewis, Mark P.
Martin, Neil R.W.
Player, Darren J.
Taylor, Conor W.
Turner, Mark C.
|Issue Date: ||2018|
|Publisher: ||© European College of Sport Science. Published by Taylor and Francis|
|Citation: ||FERGUSON, R.A. ... et al., 2018. The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction. European Journal of Sport Science, In Press.|
|Abstract: ||This study investigated protein kinase activation and gene expression of angiogenic factors in response to low-load resistance exercise with or without blood flow restriction (BFR). In a repeated measures cross-over design, six males performed four sets of bilateral knee extension exercise at 20% 1RM (reps per set = 30:15:15:continued to fatigue) with BFR (110 mmHg) and without (CON). Muscle biopsies were obtained from the vastus lateralis before, 2 and 4 h post-exercise. mRNA expression was determined using real-time RT-PCR. Protein phosphorylation/expression was determined using Western blot. p38MAPK phosphorylation was greater (p = 0.05) at 2 h following BFR (1.3 ± 0.8) compared to CON (0.4 ± 0.3). AMPK phosphorylation remained unchanged. PGC-1α mRNA expression increased at 2 h (5.9 ± 1.3 vs. 2.1 ± 0.8; p = 0.03) and 4 h (3.2 ± 0.8 vs. 1.5 ± 0.4; p = 0.03) following BFR exercise with no change in CON. PGC-1α protein expression did not change following either exercise. BFR exercise enhanced mRNA expression of vascular endothelial growth factor (VEGF) at 2 h (5.2 ± 2.8 vs 1.7 ± 1.1; p = .02) and 4 h (6.8 ± 4.9 vs. 2.5 ± 2.7; p = .01) compared to CON. mRNA expression of VEGF-R2 and hypoxia-inducible factor 1α increased following BFR exercise but only eNOS were enhanced relative to CON. Matrix metalloproteinase-9 mRNA expression was not altered in response to either exercise. Acute low-load resistance exercise with BFR provides a targeted angiogenic response potentially mediated through enhanced ischaemic and shear stress stimuli.|
|Description: ||This paper is in closed access until 17th July 2019.|
|Version: ||Accepted for publication|
|Publisher Link: ||https://doi.org/10.1080/17461391.2017.1422281|
|Appears in Collections:||Closed Access (Mechanical, Electrical and Manufacturing Engineering)|
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